Amylolytic enzyme variants

Abstract

The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.

1 . A method for preparing a dough or baked product prepared from the dough comprising adding to the dough a cyclodextrin glucanotransferase variant which forms linear oligosaccharides when acting on starch in an amount effective to retard the staling of the baked product prepared from the dough.  
     
     
         2 . The method of  claim 1 , wherein the cyclodextrin glucanotransferase variant is derived from a strain of Bacillus.  
     
     
         3 . The method of  claim 1 , wherein the cyclodextrin glucanotransferase variant is derived from a strain of Brevibacterium.  
     
     
         4 . The method of  claim 1 , wherein the cyclodextrin glucanotransferase variant is derived from a strain of Clostridium.  
     
     
         5 . The method of  claim 1 , wherein the cyclodextrin glucanotransferase variant is derived from a strain of Corynebacterium.  
     
     
         6 . The method of  claim 1 , wherein the cyclodextrin glucanotransferase variant is derived from a strain of Klebsiella.  
     
     
         7 . The method of  claim 1 , wherein the cyclodextrin glucanotransferase variant is derived from a strain of Micrococcus.  
     
     
         8 . The method of  claim 1 , wherein the cyclodextrin glucanotransferase variant is derived from a strain of Thermoanaerobacter  
     
     
         9 . The method of  claim 1 , wherein the cyclodextrin glucanotransferase variant is derived from a strain of Thermoanaerobacterium.