Method of transferring at least two saccharide units with a polyglycosyltransferase

Abstract

The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.

What is claimed as new and desired to be secured by Letters Patent of the United States is:  
     
         1 . A method of transferring at least two saccharide units with a single enzyme comprising contacting an acceptor moiety and two different sugar donors with a polyglycosyltransferase.  
     
     
         2 . The method of  claim 1 , wherein said two saccharide units are N-acetylglucosamine and N-acetylgalactosamine.  
     
     
         3 . The method of  claim 1 , wherein said acceptor moiety has a galactose at a non-reducing end.  
     
     
         4 . The method of  claim 1 , wherein said polyglycosyltransferase is isolated from Neisseria.  
     
     
         5 . The method of  claim 1 , wherein said polyglycosyltransferase is isolated from  Neisseria gonorrhoeae.    
     
     
         6 . A method of transferring N-acetylgalactosamine, to an acceptor moiety, comprising contacting an N-acetylgalactosamine donor and an acceptor moiety with an N-acetylglucosaminyl transferase.  
     
     
         7 . The method of  claim 6 , wherein said N-acetylglucosaminyl transferase is isolated from Neisseria.  
     
     
         8 . The method of  claim 6 , wherein said N-acetylglucosaminyl transferase is isolated from  Neisseria gonorrhoeae.    
     
     
         9 . The method of  claim 6 , wherein said acceptor moiety has a galactose at a non-reducing end.  
     
     
         10 . The method of  claim 8 , wherein said  Neisseria gonorrhoeae  is ATCC 33084.