Processing of chemicals in flow-through device with porous media

Abstract

A method of processing a sample comprising introducing a sample into a flow-through device containing a porous solid media therein, and thereafter subjecting the device to microwave energy.

What is claimed is:  
     
         1 . A method of processing a sample comprising 
 introducing a sample into a flow-through device containing a porous solid media therein, and    thereafter subjecting the device to microwave energy.    
     
     
         2 . The method of  claim 1  wherein said introducing said sample includes introducing said sample with a solvent to said flow-through device.  
     
     
         3 . The method of  claim 2  wherein said solvent is evaporated during said subjecting the device to microwave energy.  
     
     
         4 . The method of  claim 3  wherein said media is a chromatographic media.  
     
     
         5 . The method of  claim 1  wherein said sample includes reagents that undergo a chemical reaction to form a reaction product during said subjecting the device to microwave energy.  
     
     
         6 . The method of  claim 5  wherein said solid media includes scavengers attached to said solid media to remove excess reagents.  
     
     
         7 . The method of  claim 6  wherein said scavengers are electrophile scavengers.  
     
     
         8 . The method of  claim 7  wherein said electrophile scavengers are selected from the group consisting of amino scavengers, TsNHNH 2  scavengers, and SH scavengers.  
     
     
         9 . The method of  claim 6  wherein said scavengers are nucleophile scavengers.  
     
     
         10 . The method of  claim 9  wherein said nucleophile scavengers are selected from the group consisting of TsCl scavengers and NCO scavengers.  
     
     
         11 . The method of  claim 6  wherein said scavengers are base scavengers.  
     
     
         12 . The method of  claim 11  wherein said base scavenger is a quaternary amine scavenger.  
     
     
         13 . The method of  claim 6  wherein said scavengers are acid scavengers.  
     
     
         14 . The method of  claim 13  wherein said acid scavengers are selected from the group consisting of TsOH scavengers and COOH scavengers.  
     
     
         15 . The method of  claim 5  wherein said solid media includes coupling agents attached to said solid media.  
     
     
         16 . The method of  claim 15  wherein said coupling agents are selected from the group consisting of DCC coupling agents, HOBt coupling agents, and NHS coupling agents.  
     
     
         17 . The method of  claim 5  wherein said solid media includes a catalyst attached to said solid media.  
     
     
         18 . The method of  claim 17  wherein said catalyst is TSOH.  
     
     
         19 . The method of  claim 5  wherein said solid media includes a catalyst remover attached to said solid media.  
     
     
         20 . The method of  claim 19  wherein said catalyst remover is DEAM.  
     
     
         21 . A method of processing a sample comprising 
 introducing a sample into a flow-through device containing a porous solid media therein and active components attached to said solid media, and    thereafter subjecting the device to microwave energy, heat or UV energy in order to accelerate reactions implemented by said active components, said reactions resulting in a reaction product created from said sample.    
     
     
         22 . The method of  claim 21  further comprising thereafter transferring said reaction product from said flow-through device to a chromatography column.  
     
     
         23 . A method of processing a sample comprising 
 introducing reagents into a flow-through device containing a porous solid media therein and active components attached to said solid media,    causing a synthesis reaction involving said reagents in said flow-through device and resulting in a reaction product,    thereafter placing said flow-through device into an entrance region within a chromatography column, and    thereafter carrying out chromatography on said reaction product.    
     
     
         24 . The method of  claim 23  further comprising subjecting the device to microwave energy during said causing step.  
     
     
         25 . A chromatography sample module comprising 
 a flow-through member having an inlet and an outlet, and    a chromatography media prepacked within said flow-through member, treated with a sample dissolved in a solution, and dried, resulting in a dried sample on said chromatography media, said chromatography media being spaced from said inlet, so that said flow-through member defines a receiving region adapted to receiving a head piece for making a seal with said flow-through member,    said chromatography media including attached active components.    
     
     
         26 . A chromatography sample module comprising a tubular member that is sized to fit entirely within the end of a chromatography column containing a separation media carrying a sample, said module having an inlet and an outlet, and chromatography media within said tubular member and spaced from said inlet, so that said tubular member defines a receiving region adapted to receive a head piece, said tubular member sized to be sealed within said chromatography column with a sealing device used to seal said chromatography column, 
 said chromatography media including attached active components.    
     
     
         27 . A chromatography sample module comprising 
 a flow-through member having walls and having an inlet end and an outlet end;    a media disposed within said flow-through member, said media being spaced from said inlet end so that said walls extend above said media and so that said flow-through member defines a receiving region adapted to receive a head piece; and    a sample carried on said media,    said chromatography media including attached active components.    
     
     
         28 . The method of  claim 21 ,  22 ,  23 , or  24  wherein said active components are at least one member selected from the group consisting of scavengers, coupling agents, catalysts, and catalyst removers.  
     
     
         29 . The module of claims  26  or  27  wherein said active components are at least one member selected from the group consisting of scavengers, coupling agents, catalysts, and catalyst removers.  
     
     
         30 . A method of processing a sample comprising 
 introducing a sample into a flow-through device containing a porous solid media therein, and    thereafter subjecting the device to a radiated energy source.    
     
     
         31 . The method of  claim 30  wherein said radiated energy source provides microwave energy.  
     
     
         32 . The method of  claim 30  wherein said radiated energy source provides ultra-violet energy.  
     
     
         33 . The method of  claim 30  wherein said radiated energy source provides sonic energy.  
     
     
         34 . A method of processing a sample comprising 
 introducing a sample into a flow-through device containing a porous solid media therein and active components attached to said solid media, and    thereafter subjecting the device to energy in order to accelerate or promote reactions implemented by said active components, said reactions resulting in a reaction product created from said sample.    
     
     
         35 . A method of processing a sample comprising 
 introducing a sample into a flow-through device containing a porous solid media therein and active components attached to said solid media, said device having a liquid receiving region above said media,    carrying out a reaction on said sample using said active components, and    adding wash solvent to said liquid receiving region.    
     
     
         36 . A method of processing a sample comprising 
 introducing reagents into a flow-through device containing a porous solid media therein and active components attached to said solid media,    causing a chemical reaction involving said reagents in said flow-through device and resulting in a reaction product,    thereafter placing said flow-through device into fluid flow communication with a chromatography column, and    thereafter carrying out chromatography on said reaction product.    
     
     
         37 . The method of  claim 36  further comprising subjecting the device to energy in order to accelerate or promote said chemical reaction.  
     
     
         38 . The method of  claim 36  wherein said device is placed into said chromatography column.  
     
     
         39 . The method of  claim 36  wherein said device is connected to said chromatography column by a tube.  
     
     
         40 . A sample processing module comprising 
 a tubular flow-through member that is sized to fit entirely within the end of a chromatography column containing a separation media carrying a sample, said module having an inlet and an outlet, and media within said tubular member, said chromatography media including attached active components.    
     
     
         41 . A chromatography sample module comprising 
 a flow-through member having walls and having an inlet end and an outlet end;    a media disposed within said flow-through member; and    a sample within said flow through member,    said media including attached active components.    
     
     
         42 . The module of  claim 40  or  41  further comprising a porous frit in said flow-through member to contain said media.  
     
     
         43 . The module of  claim 40  or  41  further comprising a porous frit in said flow-through member to contain said media, said frit being flush with said inlet end or said outlet end.  
     
     
         44 . The module of  claim 40  or  41  wherein said flow through member has a liquid receiving region between said media and said inlet to enable wash solvent to be added to said flow through member.