Method and reagent for inhibiting P-glycoprotein (mdr-1 gene)

Abstract

An enzymatic RNA molecule which specifically cleaves mRNA encoded by an mdr-1 gene.

1 . An enzymatic RNA molecule which is active to specifically cleave mRNA expressed from an mdr 1 gene.  
     
     
         2 . The enzymatic RNA molecule of  claim 1  which cleaves the sequence shown as any of SEQ. ID. NOS. 1-9.  
     
     
         3 . The enzymatic RNA molecule of  claim 1  wherein said RNA molecule is in a hammerhead motif.  
     
     
         4 . The enzymatic RNA molecule of  claim 1  wherein said RNA molecule is in a hairpin, hepatitis Delta virus, group 1 intro, or RnaseP RNA motif.  
     
     
         5 . A mammalian cell including an enzymatic RNA molecule of  claim 1 .  
     
     
         6 . A mammalian cell including an enzymatic RNA molecule of  claim 2 .  
     
     
         7 . A mammalian cell including an enzymatic RNA molecule of  claim 3 .  
     
     
         8 . A mammalian cell including an enzymatic RNA molecule of  claim 4 .  
     
     
         9 . The cell of  claim 5 , wherein said cell is a human cell.  
     
     
         10 . The cell of  claim 6 , wherein said cell is a human cell.  
     
     
         11 . The cell of  claim 7 , wherein said cell is a human cell.  
     
     
         12 . The cell of  claim 8 , wherein said cell is a human cell.  
     
     
         13 . An expression vector including nucleic acid encoding the catalytic RNA molecule of any of  claim 1 , in a manner which allows expression of that enzymatic RNA molecule within a mammalian cell.  
     
     
         14 . An expression vector including nucleic acid encoding the catalytic RNA molecule of any of  claim 2 , in a manner which allows expression of that enzymatic RNA molecule within a mammalian cell.  
     
     
         15 . An expression vector including nucleic acid encoding the catalytic RNA molecule of any of  claim 3 , in a manner which allows expression of that enzymatic RNA molecule within a mammalian cell.