Method for detection of bromine in urine using liquid chemistry, dry chemistry test pads. and lateral flow

Abstract

This invention is in the field of toxicology and clinical diagnostics. More specifically, this invention provides a single dry chemistry, liquid chemistry, or lateral flow dry chemistry combination test device for use in the detection of adulteration by the addition of bromine(s) to a specimen submitted for Drugs of Abuse (DAU) testing and clinical diagnostic purposes in aqueous fluids, including urine, saliva, serum, blood, sweat extracts, and liquid homogenates of hair.

I claim:  
     
         1 . The method for the detection of bromine in a biological sample, wherein the biological sample is selected from the group consisting essentially of urine, serum, whole blood, cerebral spinal fluid, gastric fluid, hair homogenates, sweat extracts or saliva, consisting essentially of; 
 (A) preparing a liquid chemistry test means with reagent solutions consisting essentially of an indicator and a buffer, wherein the indicator is selected from the group consisting of 1,2,3,4-tetrahydrobenzo(h)quinolin-3-ol, 1,2,3,4-tetrahydrobenzo(h)quinolone, 1,2,3,4-tetrahydrobenzo(h)quinaldine, 3-hydroxy-1,2,3,4-tetrahydrobenzo(h)quinolone, 3-hydroxy-N-methyl-1,2,3,4-tetrahydrobenzo(h)quinolone, 3-acetoxy-N-methyl-1,2,3,4-tetrahydrobenzo(h)quinolone, N-methyl-1,2,3,4-tetrahydrobenzo(h)quinolone, 1,3-phenylenediamine, 1,2,3,4-tetrahydroquinoline, 1,2,3,4-tetrahydroisoquinoline hydrochloride, 7,8-benzoquinoline, 1,2,3,4-tetrahydro-3-isoquinolinecarboxylicacid hydrochloride, 1,2,3,4-tetrahydro-1-napthylamine hydrochloride, napthylamine, N,N-dialkyl-alpha-napthylamine, phenolphthalin, 2,2′-Azino-di-(3-ethylbenzthiazolinesulfonic acid), 2,2′-Azino-di-(3-ethylbenzthiazolinesulfonic acid) diammonium salt, cyanoditoly tetrazolium chloride, 3,3′-diaminobenzidine, o-dianisidine, dimethoxybenzidine, 0-phenylenediamine, 3-amino-9-ethylcarbazole, 3,3′-5,5′-tetramethylbenzidine, dimethoxybenzidine, 8-hydroxyquinoline, m-phenylenediamine, 3-dimethylaminobenzoic acid, 5-aminosalicylic acid, 4-chloro-1-napthol, diazotizable amine, sulfanilic acid, arsanilic acid, sulfanilamide, aminobenzoic acid or 4-aminoantipyrine in combination with one of the following compounds; p-hydroxybenzene sulfonate, p-hydroxybenzoic acid, n-ethyl-n-(2-hydroxy-3-sulfopropyl)-m-toluidine, n-ethyl-n-sulfopropyl-m-toluidine, 2-hydroxy-3,5-dichlorobenzenesulfonic acid, 3-hydroxy-2,4,6-triiodobenzoic acid, and 3-hydroxy-2,4,6-tribromobenzoic acid;    (B) placing the reagent in the reagent compartment of a chemistry autoanalyzer, aliquoting samples, calibrators, and controls into sample cups and placing them on the chemistry autoanalyzer;    (C) and transferring aliquots of each sample, calibrator, and control into single discrete cuvettes mounted within the chemistry autoanalyzer;    (D) aliquoting the said reagent into each cuvette and mixing; and    (E) incubating the reaction mixture;    (F) measuring and recording absorbance values of the reaction mixtures with the chemistry autoanalyzer's spectrophotometer at 340 nm to 800 nm at preprogrammed time intervals; and    (G) comparing absorbance values of samples and controls to those of calibrators in the form of a standard curve thereby quantitating the bromine present.    
     
     
         2 . The method for the detection of bromine in a biological sample, wherein the biological sample is selected from the group consisting essentially of urine, serum, whole blood, cerebral spinal fluid, gastric fluid, hair homogenates, sweat extracts or saliva, consisting essentially of; 
 (A) preparing a liquid chemistry test means with reagent solutions consisting essentially of an indicator and a buffer, wherein the buffer is selected from the group consisting of citrate, borate, borax, sodium tetraborate decahydrate, sodium perchlorate, sodium chlorate, sodium carbonate, (Tris[hydroxymethyl]aminomethane), (2-[N-Morpholino]ethanesulfonic acid), (bis[2-Hydroxyethyl]iminotris[hydroxymethyl]methane; 2-bis[2-hydroxyethyl]amino-2-[hydroxymethyl-1,3-propanediol), (N-[2-Acetamidol]-2-iminodiacetic acid; N-[Carbaoylmethyl]iminodiacetc acid), (2-[(2-Amino-2-oxoethyl)amino]ethanesulfonic acid; N-[2-Acetamido]-2-aminoethanesulfonic acid), (PiperazineN-N′-bis[2-ethanesulfonic acid)]; 1,4-Piperzinedethanesulfoic acid), (3-[N-Morpholinol]-2-hydroxypropanesulfonic acid), (1,3-bis[tris(Hydroxymethyl)methylamino]propane), (N,N-bis[2-Hydroxyethyl]-2-aminoethaesulfonic acid; 2-bis(2-Hydroxyethyl)amino]ethanesulfonic acid), (3-[N-Morpholino]propanesulfonic acid), (N-tris[Hydroxymethyl]methyl-2-aminomethanesulfonic acid; 2[2-Hysroxy-1,1-bis(hydroxymethyl)-ethyl]amino)ethanesulfonic acid), (3-[N,N-bis(2-Hydroxyethyl)amino]-2-hydroxypropanesulfonic acid), (3-[N-tris(Hydroxyethyl)methylamino]-2-(hydroxypropanesulfonic acid), (N-[2-Hydroxythyl]piperazine-N′-[2Hydroxypropanesulfonic acid]), (Piperazine-N,N′-bis[2-hydroxypropanesulfonic acid]), (N-[2-Hydroxyethyl]piperazine-N′-[3-propanesulfonic acid), (triethanolamine), (N-tris[Hydroxymethyl]methyllycine; N-[2-Hydroxy-1-1-bis(hydroxymethyl)etyyl]glycine), (N,N-bis[2-Hydroxyethyl]glycine), (N-tris[Hydroxymethyl]methyl-3-aminopropanesulfonic acid; ([2-Hdroxy-1,1-bis(hydroxymethyl)ethyl]amino)-1-propanesulfonic acid), (3-[(1,1-Dimethyl-2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid), (2-[N-Cyclohexylamino]ethanesulfonic acid), (3-[Cyclohexylamino]-2-hydroxy-1-propanesulfonic acid), 2-Amino-2-ethyl-1-propanol, (3-[cyclohexylamino]-1-propanesulfonic acid), hydrochloric acid, phosphoric acid, lactic acid, sulfuric acid, nitric acid, chromic acid, boric acid, citric acid, oxalic acid, tartaric acid, succinic acid, perchloric acid, potassium hydrogen tartrate, potassium hydrogen phthalate, calcium hydroxide, phosphate, bicarbonate, sodium hydroxide, potassium hydroxide, tartrate, oxalate or succinate;    (B) placing the reagent in the reagent compartment of a chemistry autoanalyzer, aliquoting samples, calibrators, and controls into sample cups and placing them on the chemistry autoanalyzer;    (C) and transferring aliquots of each sample, calibrator, and control into single discrete cuvettes mounted within the chemistry autoanalyzer;    (D) aliquoting the said reagent into each cuvette and mixing; and    (E) incubating the reaction mixture;    (F) measuring and recording absorbance values of the reaction mixtures with the chemistry autoanalyzer's spectrophotometer at 340 nm to 800 nm at preprogrammed time intervals; and    (G) comparing absorbance values of samples and controls to those of calibrators in the form of a standard curve thereby quantitating the bromine present.