Targeted recombinant viral vectors

Abstract

The present invention provides methods and a composition for targeted delivery of a nucleic acid to a cell comprising a biotinylated recombinant encapsidated virus, particularly adenovirus, wherein the recombinant adenovirus comprises the nucleic acid, and wherein the biotinylated recombinant virus is linked via streptavidin to a biotinylated targeting moiety.

What is claimed is:  
     
         1 . A composition for targeted delivery of a nucleic acid to a cell comprising a biotinylated recombinant adenovirus, wherein the recombinant adenovirus comprises the nucleic acid, and wherein the recombinant adenovirus is linked via streptavidin to a biotinylated targeting moiety.  
     
     
         2 . The composition of  claim 1 , wherein the targeting moiety is a ligand.  
     
     
         3 . The composition of  claim 1 , wherein the targeting moiety is an antibody.  
     
     
         4 . The composition of  claim 1 , wherein the targeting moiety is steel factor.  
     
     
         5 . The composition of  claim 1 , wherein the targeting moiety is an antibody directed against cell surface markers for steel factor.  
     
     
         6 . The composition of  claim 1 , wherein the targeting moiety is antibody anti-CD34.  
     
     
         7 . The composition of  claim 1 , wherein the targeting moiety is antibody anti-CD44.  
     
     
         8 . The composition of  claim 1 , wherein the targeting moiety is antibody anti-CD117.  
     
     
         9 . The composition of  claim 1 , wherein the nucleic acid encodes a cytotoxic protein.  
     
     
         10 . A method for targeted delivery of a nucleic acid to a selected cell in a subject comprising administering to the subject a composition comprising a biotinylated recombinant adenovirus, wherein the recombinant adenovirus comprises the nucleic acid, and wherein the recombinant virus is linked via streptavidin to a biotinylated targeting moiety that specifically targets the selected cell.  
     
     
         11 . The method of  claim 10 , wherein the targeting moiety is a ligand.  
     
     
         12 . The method of  claim 10 , wherein the targeting moiety is an antibody.  
     
     
         13 . The method of  claim 10 , wherein the targeting moiety is steel factor.  
     
     
         14 . The method of  claim 10 , wherein the targeting moiety is an antibody directed against cell surface markers for steel factor.  
     
     
         15 . The composition of  claim 10 , wherein the targeting moiety is antibody anti-CD34.  
     
     
         16 . The composition of  claim 10 , wherein the targeting moiety is antibody anti-CD44.  
     
     
         17 . The composition of  claim 10 , wherein the targeting moiety is antibody anti-CD117.  
     
     
         18 . The composition of  claim 10 , wherein the nucleic acid encodes a cytotoxic protein.  
     
     
         19 . A composition for targeted delivery of a nucleic acid to a cell comprising a biotinylated recombinant encapsidated virus, wherein the recombinant virus comprises the nucleic acid, and wherein the recombinant virus is linked via streptavidin to a biotinylated targeting moiety.  
     
     
         20 . The composition of  claim 19 , wherein the virus is an adenovirus.  
     
     
         21 . The composition of  claim 19 , wherein the virus is an adeno-associated virus.  
     
     
         22 . The method of  claim 19 , wherein the targeting moiety is a ligand.  
     
     
         23 . The method of  claim 19 , wherein the targeting moiety is an antibody.  
     
     
         24 . The method of  claim 19 , wherein the targeting moiety is steel factor.  
     
     
         25 . The method of  claim 19 , wherein the targeting moiety is an antibody directed against cell surface markers for steel factor.  
     
     
         26 . The composition of  claim 19 , wherein the targeting moiety is antibody anti-CD34.  
     
     
         27 . The composition of  claim 19 , wherein the targeting moiety is antibody anti-CD44.  
     
     
         28 . The composition of  claim 19 , wherein the targeting moiety is antibody anti-CD117.  
     
     
         29 . The composition of  claim 19 , wherein the nucleic acid encodes a cytotoxic protein.  
     
     
         30 . A method for targeted delivery of a nucleic acid to a selected cell in a subject comprising administering to the subject a composition comprising a biotinylated recombinant encapsidated virus, wherein the recombinant virus comprises the nucleic acid, and wherein the recombinant virus is linked via streptavidin to a biotinylated targeting moiety that specifically targets the selected cell.  
     
     
         31 . The method of  claim 30 , wherein the virus is an adenovirus.  
     
     
         32 . The method of  claim 30 , wherein the virus is an adeno-associated virus.  
     
     
         33 . The method of  claim 30 , wherein the targeting moiety is a ligand.  
     
     
         34 . The method of  claim 30 , wherein the targeting moiety is an antibody.  
     
     
         35 . The method of  claim 30 , wherein the targeting moiety is steel factor.  
     
     
         36 . The method of  claim 30 , wherein the targeting moiety is an antibody directed against cell surface markers for steel factor.  
     
     
         37 . The composition of  claim 30 , wherein the targeting moiety is antibody anti-CD34.  
     
     
         38 . The composition of  claim 30 , wherein the targeting moiety is antibody anti-CD44.  
     
     
         39 . The composition of  claim 30 , wherein the targeting moiety is antibody anti-CD117.  
     
     
         40 . The composition of  claim 30 , wherein the nucleic acid encodes a cytotoxic protein.