Diagnostic assay for cancer

Abstract

The invention is directed towards gene expression characteristic for cancer, in particular brain cancers such as glioblastoma. Compositions, methods and kits encompassing such therein.

We claim:  
     
         1 . An isolated nucleic acid molecule comprising a sequence substantially identical to SEQ ID NO: 4.  
     
     
         2 . An expression construct comprising the nucleic acid of  claim 1  operably linked to a transcriptional control region.  
     
     
         3 . A host cell comprising the expression construct of  claim 2 .  
     
     
         4 . A method of producing a protein encoded by SEQ ID NO: 4, the method comprising culturing the host cell of  claim 3  under conditions permitting expression of said protein.  
     
     
         5 . An isolated nucleic acid molecule comprising a sequence that hybridizes under stringent conditions to SEQ ID NO: 4, or the complement thereof.  
     
     
         6 . An expression construct comprising the nucleic acid of  claim 5  operably linked to a transcriptional control region.  
     
     
         7 . A host cell comprising the expression construct of  claim 6 .  
     
     
         8 . A method of producing a protein encoded by SEQ ID NO: 4, the method comprising culturing the host cell of  claim 7  under conditions permitting expression of said protein.  
     
     
         9 . An isolated nucleic acid molecule comprising a sequence substantially identical to SEQ ID NO: 6.  
     
     
         10 . An expression construct comprising the nucleic acid of  claim 9  operably linked to a transcriptional control region.  
     
     
         11 . A host cell comprising the expression construct of  claim 10 .  
     
     
         12 . A method of producing a protein encoded by SEQ ID NO: 6, the method comprising culturing the host cell of  claim 11  under conditions permitting expression of said protein.  
     
     
         13 . An isolated nucleic acid molecule comprising a sequence that hybridizes under stringent conditions to SEQ ID NO: 6, or the complement thereof.  
     
     
         14 . An expression construct comprising the nucleic acid of  claim 13  operably linked to a transcriptional control region.  
     
     
         15 . A host cell comprising the expression construct of  claim 14 .  
     
     
         16 . A method of producing a protein encoded by SEQ ID NO: 6, the method comprising culturing the host cell of  claim 15  under conditions permitting expression of said protein.  
     
     
         17 . An isolated nucleic acid molecule comprising a sequence substantially identical to SEQ ID NO: 7.  
     
     
         18 . An expression construct comprising the nucleic acid of  claim 17  operably linked to a transcriptional control region.  
     
     
         19 . A host cell comprising the expression construct of  claim 18 .  
     
     
         20 . A method of producing a protein encoded by SEQ ID NO: 7, the method comprising culturing the host cell of  claim 19  under conditions permitting expression of said protein.  
     
     
         21 . An isolated nucleic acid molecule comprising a sequence that hybridizes under stringent conditions to SEQ ID No: 7, or the complement thereof.  
     
     
         22 . An expression construct comprising the nucleic acid of  claim 21  operably linked to a transcriptional control region.  
     
     
         23 . A host cell comprising the expression construct of  claim 22 .  
     
     
         24 . A method of producing a protein encoded by SEQ ID NO: 7, the method comprising culturing the host cell of  claim 23  under conditions permitting expression of said protein.  
     
     
         25 . An isolated nucleic acid molecule comprising a sequence substantially identical to SEQ ID NO: 8.  
     
     
         26 . An expression construct comprising the nucleic acid of  claim 25  operably linked to a transcriptional control region.  
     
     
         27 . A host cell comprising the expression construct of  claim 26 .  
     
     
         28 . A method of producing a protein encoded by SEQ ID NO: 8, the method comprising culturing the host cell of  claim 27  under conditions permitting expression of said protein.  
     
     
         29 . An isolated nucleic acid molecule comprising a sequence that hybridizes under stringent conditions to SEQ ID No: 8, or the complement thereof.  
     
     
         30 . An expression construct comprising the nucleic acid of  claim 29  operably linked to a transcriptional control region.  
     
     
         31 . A host cell comprising the expression vector of  claim 30 .  
     
     
         32 . A method of producing a protein encoded by SEQ ID NO: 8, the method comprising culturing the host cell of  claim 31  under conditions permitting expression of said protein.  
     
     
         33 . A kit comprising a first probe that hybridizes to a target under stringent conditions, wherein said target is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID: 9, or complements thereof.  
     
     
         34 . The kit of  claim 33 , further comprising a second probe that hybridizes to a target under stringent conditions, wherein said target is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ 
 ID NO: 7, SEQ ID NO: 8, and SEQ ID: 9, or complements thereof.    
     
     
         35 . A kit of  claim 34 , further comprising suitable reaction buffer components.  
     
     
         36 . A kit of  claim 35 , wherein said kit is adapted for use in PCR amplification of the specific target.  
     
     
         37 . A kit as in  claim 35 , wherein said kit is adapted for use in in situ hybridization. 
 A kit of  claim 35 , wherein said kit is adapted for use in RNase protection assay.    
     
     
         38 . A DNA chip comprising nucleotide sequences capable of hybridizing to one or more members of a panel of DNA sequences selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 9, or complements thereof.  
     
     
         39 . A kit comprising probes specific for at least one protein containing an amino acid sequence corresponding to the translation of at least one nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 9.