Disease associated protein kinases

Abstract

The invention provides human disease associated protein kinases and polynucleotides (collectively designated DAPK) which identify and encode them. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention further provides methods for diagnosing and treating disorders associated with expression of human disease associated protein kinases.

What is claimed is:  
     
         1 . An isolated polynucleotide, or the complement thereof, encoding a polypeptide selected from the group consisting of: 
 a) SEQ ID NOs:2, 4, 5, 6, or 7, and    b) a naturally-occurring amino acid sequence having at least 90% sequence identity over the complete sequence of SEQ ID NOs:1, 2, 4, or 7, and which retains protein kinase activity.    
     
     
         2 . A recombinant polynucleotide comprising a promoter sequence operably linked to a polynucleotide of  claim 1 .  
     
     
         3 . A host cell transformed with the recombinant polynucleotide of  claim 2 .  
     
     
         4 . A method for producing a polypeptide, the method comprising the steps of: 
 a) culturing the host cell of  claim 3  under conditions suitable for the expression of the polypeptide; and    b) recovering the polypeptide from the host cell culture.    
     
     
         5 . A composition comprising the polynucleotide sequence of  claim 1  and a pharmaceutically acceptable excipient.  
     
     
         6 . An isolated polynucleotide sequence selected from SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:14, or the complement thereof.  
     
     
         7 . A composition comprising the polynucleotide sequence of  claim 6  and a pharmaceutically acceptable excipient.  
     
     
         8 . A method for detecting a polynucleotide in a sample comprising the steps of: 
 a) hybridizing the polyucleotide of  claim 6  to nucleic acids of the sample, thereby forming hybridization complexes; and    b) comparing hybridization complex formation with a standard, wherein the comparison indicates expression of the polynucleotide in the sample.    
     
     
         9 . The method of  claim 8  further comprising amplifying the nucleic acids of the sample prior to hybridization.  
     
     
         10 . The method of  claim 8  wherein the polynucleotide is attached to a substrate.  
     
     
         11 . A method of using a polynucleotide to screen a plurality of molecules or compounds for a molecule or compound which specifically binds the polynucleotide, the method comprising: 
 a) combining the polynucleotide of  claim 6  with a plurality of molecules or compounds under conditions to allow specific binding; and    b) detecting specific binding, thereby identifying a molecule or compound which specifically binds the polynucleotide.    
     
     
         12 . The method of  claim 11  wherein the molecules or compounds are selected from DNA molecules, RNA molecules, peptide nucleic acids, artificial chromosome constructions, peptides, transcription factors, and regulatory molecules.  
     
     
         13 . An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: 
 a) SEQ ID NOs:1, 2, 4, 5, 6, or 7, and    b) a naturally-occurring amino acid sequence having at least 90% sequence identity over the complete sequence of SEQ ID NOs:1,2, 4, or 7, and which retains protein kinase activity.    
     
     
         14 . A purified antibody which specifically binds to the polypeptide of  claim 13 .  
     
     
         15 . The antibody of  claim 14 , wherein the antibody is: 
 (a) a chimeric antibody;    (b) a single chain antibody;    (c) a Fab fragment;    (d) a F(ab′) 2  fragment;    (e) a Fv fragment; or    (f) a humanized antibody.    
     
     
         16 . A composition comprising an antibody of  claim 14  and a pharmaceutically acceptable excipient.  
     
     
         17 . A method of diagnosing a condition or disease associated with the expression of DAPK in a subject, comprising administering to said subject an effective amount of the composition of  claim 16 .  
     
     
         18 . A composition of  claim 16 , wherein the antibody is labeled.  
     
     
         19 . A method of diagnosing a condition or disease associated with the expression of DAPK in a subject, comprising administering to said subject an effective amount of the composition of  claim 18 .  
     
     
         20 . A method for detecting a DAPK polypeptide in a sample comprising the steps of: 
 a) combining the antibody of  claim 14  with a sample under conditions to allow specific binding; and    b) detecting specific binding, wherein specific binding indicates the presence of the DAPK polypeptide in the sample.    
     
     
         21 . A method of using an antibody to purify a DAPK polypeptide from a sample, the method comprising: 
 a) combining the antibody of  claim 14  with a sample under conditions to allow specific binding; and    b) separating the antibody from the protein, thereby obtaining purified DAPK polypeptide.