Galanin receptor, nucleic acids, transformed cell and uses thereof

Abstract

Polypeptides having galinin receptor activity, genetic material for expressing same, any recombinant cell expressing said polypeptides, and the use thereof, are disclosed.

1 . A nucleotide sequence selected from the group consisting of: 
 (a) all or part of the nucleotide sequence SEQ ID No. 1 or of its complementary strand,    (b) a sequence hybridizing with a sequence (a) and coding for a polypeptide having galaninergic receptor activity, and    (c) the sequences derived from the sequences (a) and (b) as a result of the degeneracy of the genetic code.    
     
     
         2 . A nucleotide sequence according to  claim 1 , wherein said sequence encodes a human galaninergic receptor.  
     
     
         3 . An isolated nucleotide sequence comprising all or part of the nucleotide sequence SEQ ID No. 1 or of its complementary strand.  
     
     
         4 . A nucleotide sequence according to  claim 1  comprising a region encoding a polypeptide under the control of signals permitting expression of the polypeptide in a cell host.  
     
     
         5 . An isolated polypeptide comprising all or part of the peptide sequence SEQ ID No. 2 or of a derivative thereof.  
     
     
         6 . An isolated polypeptide encoded by a nucleotide sequence selected from the group consisting of 
 (a) all or part of the nucleotide sequence SEQ ID No. 1,    (b) a sequence hybridizing with a sequence (a) and coding for a polypeptide having galaninergic receptor activity, and    (c) nucleotide sequences encoding the amino acid sequences encoded by (a) or (b).    
     
     
         7 . An antisense sequence capable of at least partially inhibiting the production of a polypeptide according to  claim 6 .  
     
     
         8 . A polypeptide according to  claim 6  possessing galaninergic receptor activity.  
     
     
         9 . A recombinant cell expressing at its surface a polypeptide according to  claim 6 .  
     
     
         10 . A cell according to  claim 9 , wherein said cell is eukaryotic.  
     
     
         11 . A ligand or modulator for a polypeptide as defined in  claim 6 .  
     
     
         12 . A pharmaceutical composition comprising as active principle at least one molecule which acts on a polypeptide according to  claim 6 .  
     
     
         13 . A pharmaceutical composition according to  claim 12 , wherein the molecule is a ligand or a modulator of said polypeptide.  
     
     
         14 . A method for demonstrating and/or isolating ligands for galaninergic receptors, said method comprising contacting a molecule or a mixture containing different molecules with a recombinant cell according to  claim 13  under conditions permitting interaction between the said polypeptide and the said molecule.  
     
     
         15 . A method according to  claim 14 , wherein said ligands are agonists and/or antagonists for the galaninergic receptors.  
     
     
         16 . A method for demonstrating and/or isolating modulators for galaninergic receptors, said method comprising contacting a molecule or a mixture containing different molecules with a recombinant cell according to  claim 9  under conditions permitting interaction between the said polypeptide and the said molecule.  
     
     
         17 . A diagnostic assay comprising a step of hybridizing a nucleic acid according to  claim 1  to a homologous nucleic acid sequence.