Novel gene

Abstract

The invention provides an isolated inflammation-related gene, the protein molecule encoded by the gene, and the use of the gene in diagnosis and treatment of inflammatory conditions.

1 . An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:19 or SEQ ID NO:20 or a functionally equivalent variant of said amino acid sequence.  
     
     
         2 . An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or a functionally equivalent variant of said amino acid sequence.  
     
     
         3 . An isolated polynucleotide according to  claim 1  which is cDNA comprising the nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:16 or SEQ ID NO:17 or SEQ ID NO:18, or a DNA comprising a nucleotide sequence which hybridises to SEQ ID NO:1 or SEQ ID NO:16 or SEQ ID NO:17 or SEQ ID NO:18 under stringent conditions.  
     
     
         4 . An isolated polynucleotide according to  claim 1  which is cDNA comprising the nucleotide sequence of SEQ ID NO:1, or a DNA comprising a nucleotide sequence which hybridises to SEQ ID NO:1 under stringent conditions.  
     
     
         5 . An isolated polynucleotide comprising a consecutive 20 base pair nucleotide portion identical in sequence to a consecutive 20 base pair portion of SEQ ID NO:1.  
     
     
         6 . An isolated polynucleotide comprising a nucleotide sequence encoding at least 10 contiguous amino acids from SEQ ID NO:2 or SEQ ID NO:19 or SEQ ID NO:20.  
     
     
         7 . An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:19 or SEQ ID NO:20 or a functionally equivalent variant thereof.  
     
     
         8 . An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2 or a functionally equivalent variant thereof.  
     
     
         9 . An isolated polypeptide comprising a consecutive 10 amino acid portion identical in sequence to a consecutive 10 amino acid portion of SEQ ID NO:2 or SEQ ID NO:19 or SEQ ID NO:20.  
     
     
         10 . An isolated polypeptide comprising a consecutive 10 amino acid portion identical in sequence to a consecutive 10 amino acid portion of SEQ ID NO:2.  
     
     
         11 . A method of producing a polypeptide which comprises culturing a host cell containing an expression vector containing a polynucleotide sequence as specified in  claim 1 , under conditions suitable for expression of the polypeptide and recovering the polypeptide from the host cell culture.  
     
     
         12 . An antibody which is immunoreactive with a polypeptide according to  claim 7 .  
     
     
         13 . An antisense oligonucleotide comprising a nucleotide sequence complementary to that of a polynucleotide encoding a polypeptide according to  claim 7 .  
     
     
         14 . A polynucleotide probe comprising at least 15 contiguous nucleotides of a polynucleotide according to  claim 1 , or a complement thereof.  
     
     
         15 . A pharmaceutical composition comprising a polynucleotide according to  claim 1 , a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:19 or SEQ ID NO:20 or a functionally equivalent variant thereof, an antibody which is immunoreactive with said polypeptide, a polynucleotide probe comprising at least 15 contguous nucleotides of a polynucleotide according to  claim 1  or a complement thereof, or an antisense oligonucleotide comprising a nucleotide sequence complementary to that of a polynucleotide according to  claim 1 , optionally together with a pharmaceutically acceptable carrier.  
     
     
         16 . A method of treating an inflammatory disease which comprises administering to a subject in need thereof an effective amount of a polynucleotide according to  claim 1 , a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:19 or SEQ ID NO:20 or a functionally equivalent variant thereof, an antibody which is immunoreactive with said polypeptide or an antisense oligonucleotide comprising a nucleotide sequence complementary to that of said polynucleotide.  
     
     
         17 . A method of identifying a substance which modulates the activity of a polypeptide according to  claim 7  comprising combining a candidate substance with a polypeptide according to  claim 7  and measuring the effect of the candidate substance on said activity.  
     
     
         18 . A method of detecting genetic abnormality in a subject which comprises incubating a genetic sample from the subject with a polynucleotide probe according to  claim 14 , under conditions where the probe hybridises to complementary polynucleotide sequence, to produce a first reaction product, and comparing the first reaction product to a control reaction product obtained with a normal genetic sample, where a difference between the first reaction product and the control reaction product indicates a genetic abnormality in the subject or a predisposition to developing a disease.  
     
     
         19 . A method of detecting the presence of a polynucleotide according to  claim 1  in a cell or tissue which comprises contacting DNA from the cell or tissue with a polynucleotide probe comprising at least 15 contiguous nucleotides of a polynucleotide according to  claim 1  under conditions where the probe is specifically hybridizable with a polynucleotide according to  claim 1 , and detecting whether hybridization occurs.  
     
     
         20 . A pair of oligonucleotides useful as primers for amplification of a fragment of a polynucleotide according to  claim 1 , each oligonucleotide of said pair being at least 15 nucleotides in length and said pair having sequences such that when used in a polymerase chain reaction with human genomic DNA or a suitable human cDNA target, they result in synthesis of a DNA fragment containing part or all of the nucleotide sequence of a polynucleotide according to  claim 1.