Polymer coated surfaces for microarray applications

Abstract

Methods are provide for modifying a solid support, such as a glass slide, by silylating with an agent having the formula H 2 N—(CH 2 ) n —SiX 3 where n is between 1 and 10, and X is independently chosen from OMe, OEt, Cl, Br, or I, then activating with a crosslinking reagent, followed by reacting with an amine-containing polymer. The support can optionally be reacted with a crosslinking reagent again. The support thus modified may be used to make arrays and microarrays where a plurality of targets are stably associated with the support and arranged in a defined manner.

What is claimed is:  
     
         1 . An array of attached target molecules, comprising: 
 a silylated surface of a solid support, said silylated surface being silylated with an agent having the formula H2N—(CH2)n-SiX3 where n is between 1 and 10, and X is independently chosen from OMe, OEt, Cl, Br or I: 
 a first cross-linking reagent;  
 an amine-containing polymer that forms a modified solid support surface with said silylated surface and first crossing-linking reagent; and  
 non-activated target molecules attached to said modified surface, wherein the target molecules are arranged in a defined manner on the modified surface thereby producing an array of attached target molecules.  
   
     
     
         2 . The array of  claim 1 , wherein the solid support surface is further modified with a second crosslinking reagent, and wherein target molecules are covalently attached to the modified solid support surface.  
     
     
         3 . The array of  claim 1 , wherein the solid support surface is selected from the group consisting of glass, silica, plastic, ceramic, beads, and nylon, and combinations thereof.  
     
     
         4 . The array of  claim 1 , wherein the solid support surface is glass.  
     
     
         5 . The array of  claim 1 , wherein the crosslinking reagent is cyanuric chloride.  
     
     
         6 . The array of  claim 1 , wherein the amine-containing polymer is selected from the group consisting of polyethylenimine, polyhistidine, polylysine, and polyarginine, and combinations thereof.  
     
     
         7 . The array of  claim 1 , wherein the amine-containing polymer is polyethylenimine.  
     
     
         8 . The array of  claim 1 , wherein the target molecules are selected from the group consisting of target polynucleotides, target polypeptides and target polysaccharides.  
     
     
         9 . The array of  claim 1 , wherein the target molecules are target polypeptides.  
     
     
         10 . The array of  claim 1 , wherein the target molecules are target polypeptides, the solid support surface is glass, the crosslinking reagent is cyanuric chloride, and the amine-containing polymer is polyethylenimine.  
     
     
         11 . The array of  claim 1 , wherein the target molecules are target antibodies.  
     
     
         12 . The array of  claim 1 , wherein the target molecules are target antibodies, the solid support surface is glass, the crosslinking reagent is cyanuric chloride, and the amine-containing polymer is polyethylenimine.  
     
     
         13 . The array of  claim 1 , wherein the target molecules are target monoclonal antibodies.  
     
     
         14 . The array of  claim 1 , wherein the target molecules are target monoclonal antibodies, the solid support surface is glass, the crosslinking reagent is cyanuric chloride, and the amine-containing polymer is polyethylenimine.  
     
     
         15 . The array of  claim 2 , wherein the second crosslinking reagent is cyanuric chloride.  
     
     
         16 . The array of  claim 1 , wherein the target molecules are not covalently attached to the modified solid support surface.  
     
     
         17 . The array of  claim 1 , wherein the target molecules are not covalently attached to the modified solid support surface, the solid support surface is a glass slide, and the attaching step produces a microarray of the target molecules on the slide.