Parallel production of high density arrays

Abstract

Internet communication technology is combined with global position systems (GPS) to provide anyone with access to the Internet or web to locate and determine conditions relating to a specific unit or vehicle or fleet of units or vehicles anywhere in the world. The unit or vehicle may communicate directly with the web for location based or location valuable information. Communication between units and/or vehicles is also provided. A transceiver device is located onboard the vehicle/unit and can be accessed by a local Internet interface via common carrier such as cellular telephone or the like. The transceiver includes an integral GPS signal generator and other identifying data. Once the transceiver is accessed by the Internet, the identifying data and the GPS signal are transmitted via the transceiver to the web, where it may be reviewed by any party having authorized access to the web site containing the information. The system provides instant identification of the location of any monitored unit or vehicle anywhere in the world through a local access site. The transceiver may be linked to an onboard processor, which may be a limited, dedicated processor or an expanded general purpose processor, whereby the transceiver can send and receive commands which are processed by the processor to initiate or report certain actions or conditions.

What is claimed is:  
     
         1 . A method for the detection of components in a sample mixture or detection of components on an array comprising the steps of: 
 a. immobilizing binding compounds onto a carrier;    b. forming a bundle of compounds in a generally elongated form by collecting the immobilized compounds about a common axis to form a bundle; and    c. cutting individual arrays from the bundle to generate a spatially uniform arrangement of compound samples and carrier.    
     
     
         2 . The method of  claim 1 , wherein the immobilizing step comprises immobilization of binding compounds onto rod shaped elements and wherein after the rods are formed in a bundle, there is included the step of placing a sheath around the bundle to secure the rods.  
     
     
         3 . The method of  claim 2 , wherein said sheath is an adhesive layer.  
     
     
         4 . The method of  claim 2 , wherein said sheath is a heat shrink material applied in circumscribing relationship around the bundle and heated to shrink fit.  
     
     
         5 . The method of  claim 1 , wherein the immobilizing step comprises forming lines of immobolized binding compounds on a sheet of material and the bundle is formed by the step of wrapping the sheet material in a spiral.  
     
     
         6 . The method of  claim 5 , wherein wrapping step includes rolling the sheet about a rod.  
     
     
         7 . The method  claim 1 , wherein the immobilizing step comprises synthesis of binding compounds onto rod shaped elements using a randomized method and the sub-step of marking the identification of each rod at each synthesis step.  
     
     
         8 . The method of  claim 2 , wherein sub-step of marking is by spin coating with paint.  
     
     
         9 . The method of  claim 1 , wherein the forming step includes grouping rod elements treated with a given binding agent for creating a graphic symbol(s) surrounded by rod elements with different affinity for the components in the test sample.  
     
     
         10 . The method of  claim 1 , wherein the elongated bundle is formed by stacking flat components along a common axis.  
     
     
         11 . The method of  claim 1 , the immobilizing step comprising forming lines of immobilized binding compounds on sheets of carrier material wherein the bundle is formed by alternating layers of carrier sheets and adhesive bonding layers.  
     
     
         12 . The method of  claim 11 , wherein the bundle is spiral wound.  
     
     
         13 . The method of  claim 11 , wherein the bundle is a static sheet structure.  
     
     
         14 . The method of  claim 1 , further including the steps of: 
 a. applying a sample to the surface of the array;    b. applying a washing solution to remove unbound compounds of the sample mixture;    c. applying a labeled compound that binds to the components of the sample mixture which are bound to elements of the array;    d. washing unbound label compound;    e. observing the binding pattern; and    f. correlating the binding pattern of the binding surfaces with the composition of the elements in the array.    
     
     
         15 . The method of  claim 1 , further including the step of: 
 a. cutting individual arrays from the bundle to generate a multiplicity of binding surfaces and placing one side of the array to a pad.    
     
     
         16 . The method of  claim 15 , further including the steps of: 
 a. applying a sample to the surface of an array and allowing the sample to flow through the array into the pad;    b. applying a washing solution to remove unbound compounds of the sample mixture;    c. applying a labeled compound that binds to the components of the sample mixture which are bound to elements of the array;    d. washing unbound label compound;    e. observing the binding pattern;    f. correlating the binding pattern of the binding surfaces with the composition of elements in the array.    
     
     
         17 . The method of  claim 16 , wherein the observing step includes applying a reagent to allow detection of the bound label.  
     
     
         18 . The method of  claim 5 , further including the step of separating lines of binding compounds by identification marks wherein the binding pattern on the array may be correlated with the composition of the binding elements.  
     
     
         19 . The method of  claim 5 , wherein the binding material is impregnated into the thickness of the sheet material.  
     
     
         20 . The method of  claim 18 , wherein the cutting step comprises cutting individual arrays from the bundle to expose binding elements formed by the freshly exposed edge of sheet material and separated by identification marks and interstitial space between adjacent membrane layers.  
     
     
         21 . The method of  claim 20 , further including the step of placing one side of the array onto a pad.  
     
     
         22 . The method of  claim 21 , wherein said pad is absorbent.  
     
     
         23 . The method of  claim 22 , further comprising the steps of: 
 a. cutting individual arrays from the bundle to generate a multiplicity of binding surfaces and placing one side of the array to a pad.    
     
     
         24 . The method of  claim 23 , further including the steps of: 
 a. applying a sample to the surface of an array and allowing the sample to flow through the array into the pad;    b. applying a washing solution to remove unbound compounds of the sample mixture;    c. applying a labeled compound that binds to the components of the sample mixture which are bound to elements of the array;    d. washing unbound label compound;    e. observing the binding pattern;    f. correlating the binding pattern of the binding surfaces with the composition of elements in the array.    
     
     
         25 . The method of  claim 9 , further comprising the steps of: 
 a. cutting individual arrays from the bundle and placing one side of the array to a pad;    b. applying a mixture of sample and label to the surface of the array, allowing the mixture to flow through the rod elements of the array into the pad for forming attachments between the label, analyte and immobilized binding agent as in a sandwich assay format;    c. washing away unbound label;    d. observing the binding pattern;    e. correlating the graphic symbol(s) with the presence or absence of analyte(s).    
     
     
         26 . The method of  claim 25 , wherein the observing step includes applying a reagent to allow detection of the bound label.  
     
     
         27 . A method for detection of compounds on an array by: 
 a. immobilizing binding compounds onto rod shaped array elements;    b. forming a bundle of the rod elements using a guide to create a spatially uniform arrangement of rod elements and securing with a sheath material;    c. cutting individual arrays from the bundle to generate a multiplicity of binding surfaces and fixing or placing one side of the array to an absorbent pad;    d. applying a sample to the surface of an array and allowing the sample to flow through the porous rod elements of the array into the absorbent pad;    e. exposing and developing the array;    f. correlating the binding pattern of the binding surfaces with the composition of elements in the array.    
     
     
         28 . A method of detecting components in a sample mixture or detection of compounds on an array by: 
 a. forming lines of immobilized binding compounds on a sheet of material, wherein lines of binding compounds maybe separated by identification marks, and the binding material is impregnated into the thickness of the sheet material;    b. rolling the printed sheet into spiral wound structure about a rod and securing roll with a sheath;    c. cutting individual arrays from the bundle to expose binding elements formed by the freshly exposed edge of sheet material separated by identification marks and interstitial space between adjacent membrane layers, and fixing or placing one side of the array onto an absorbent pad;    d. exposing and develop the array;    e. correlating the binding pattern on the array with the composition of the binding elements.    
     
     
         29 . A method of detecting components in a sample mixture or detection of compounds on an array by: 
 a. synthesizing of binding compounds onto rod shaped array elements using a randomized method;    b. marking the identification of each rod at each synthesis step by spin coating with paint;    c. forming a bundle of the rod elements and securing with a sheath material;    d. exposing and developing the array;    e. correlating the binding events with the identification marks.    
     
     
         30 . The method of  claim 27 ,  28  or  29 , wherein the exposing and developing step includes the sub-steps of: 
 a. applying a washing solution to remove unbound compounds of the sample mixture;  
 b. applying a labeled compound that binds to the components of the sample mixture which are bound to elements of the array;  
 c. washing away unbound label and observe binding pattern directly or by application of a reagent to allow detection of the bound label.  
 
     
     
         31 . A method of detecting components in a sample mixture or detection of compounds on an array by: 
 a. immobilizing binding compounds onto rod shaped array elements;    b. forming a bundle of the rod elements and securing with a sheath material so that rod elements treated with a given binding agent are grouped to create a graphic symbol/s surrounded by rod elements with different or substantially no affinity for components in the test sample;    c. cutting individual arrays from the bundle and fixing or placing one side of the array to an absorbent pad;    d. exposing and developing the array;    e. correlating the graphic symbol/s with the presence or absence of analyte(s).    
     
     
         32 . The method of  claim 31 , wherein the exposing and developing step comprises the sub-steps of: 
 a. applying a mixture of sample and label to the surface of an array, allowing the mixture to flow through the porous rod elements of the array into the absorbent pad and form attachments between the label, analyte and immobilized binding agent as in a “sandwich assay” format;    b. washing away unbound label and observe binding patter directly or by application of a reagent to allow detection of the bound label.