Single Chain Fv constructs of anti-ganglioside GD2 antibodies

Abstract

Recombinant antibody constructs comprise the variable regions of the heavy and light chains of anti-G D2 antibodies. These antibody constructs may be coupled to a label such as a radiolabel or to a protein such as streptavidin or pro-drug converting enzymes for use in imaging or therapeutic applications. The antibody constructs may also be transduced into T cells to produce populations of T cells which target G D2 -producing tumor cells.

1 . A recombinant single chain polynucleotide comprising a region encoding the variable region of the light chain of an anti-G D2  antibody linked to a region encoding the variable region of the heavy chain of an anti-G D2  antibody.  
     
     
         2 . The recombinant polynucleotide of  claim 1 , further comprising a region encoding an additional protein.  
     
     
         3 . The recombinant polynucleotide of  claim 1 , wherein the additional protein is streptavidin.  
     
     
         4 . The recombinant polynucleotide of  claim 1 , wherein the additional protein is a drug-converting enzyme.  
     
     
         5 . A recombinant single chain peptide comprising the variable region of the light chain of an anti-G D2  antibody linked to the variable region of the heavy chain of an anti-G D2  antibody.  
     
     
         6 . The peptide according to  claim 5 , wherein the peptide is labeled with a radiolabel.  
     
     
         7 . The peptide according to  claim 6 , wherein the radiolabel is  99m Tc.  
     
     
         8 . The peptide according to  claim 5 , wherein the peptide further comprises a drug-converting enzyme.  
     
     
         9 . The peptide according to  claim 5 , wherein the peptide further comprises streptavidin.  
     
     
         10 . The peptide according to  claim 5 , wherein the peptide further comprises CD8.  
     
     
         11 . T cells expressing a recombinant single chain peptide comprising the variable region of the light chain of an anti-G D2  antibody linked to the variable region of the heavy chain of an anti-G D2  antibody.  
     
     
         12 . A method for assaying for the presence of cells expressing G D2  in tissue comprising combining the tissue with a recombinant single chain peptide comprising the variable region of the light chain of an anti-G D2  antibody linked to the variable region of the heavy chain of an anti-G D2  antibody and a detectable label.  
     
     
         13 . A method for targeted delivery of a therapeutic agent to cells expressing G D2  in tissue comprising combining the tissue with a recombinant single chain peptide comprising the variable region of the light chain of an anti-G D2  antibody linked to the variable region of the heavy chain of an anti-G D2  antibody and a therapeutic or pre-therapeutic moiety.  
     
     
         14 . The method according to  claim 13 , wherein the pre-therapeutic moiety is a pro-drug converting enzyme.  
     
     
         15 . The method according to  claim 13 , wherein the pre-therapeutic moiety is streptavidin.  
     
     
         16 . The method according to  claim 13  wherein the therapeutic moiety is a toxin.