Glycosulfopeptide inhibitors of leukocyte rolling and methods of use thereof

Abstract

Compounds, compositions and methods for treating conditions characterized by leukocyte rolling are described. The compounds contain glycosulfopeptide structures comprising sulfated tyrosines and sialyated, fucosylated N-acetyllactosamino glycans. The glycosulfopeptides may be conjugated or complexed to other compounds for enhancing serum half-life or for controlled release, for example. Examples of conditions treated include inflammation, ischemia-reperfusion injury, rheumatoid arthritis, atherosclerosis, leukocyte-mediated lung injury, restenosis, and thrombosis.

What is claimed is:  
     
         1 . A method of inhibiting or reducing leukocyte rolling in a subject in vivo, comprising: 
 administering to the subject an effective amount of a compound comprising a glycosulfopeptide thereby inhibiting or reducing leukocyte rolling in the subject, the glycosulfopeptide comprising the structure:                          wherein C is an O-, N-, or S-linking amino acid; and    wherein R is one of R 1 , R 3 -R 13  or R 15 , wherein:                                                                                
     
     
         2 . The method of  claim 1  wherein C is serine, threonine, hydroxyproline, tyrosine, hydroxylysine, methionine, cysteine, lysine, asparagine, or glutamine.  
     
     
         3 . The method of  claim 1  wherein the glycosulfopeptide is combined with a polymer to form the compound.  
     
     
         4 . A composition for inhibiting or reducing leukocyte rolling in a subject in vivo, comprising: 
 a pharmaceutically acceptable carrier; and    a compound comprising a glycosulfopeptide comprising the structure:                          wherein, C is an O-, N-, or S-linking amino acid; and    wherein, R is one of R 1 , R 3 -R 13  or R 15  wherein:                                                                                
     
     
         5 . The composition of  claim 4  wherein C is serine, threonine, hydroxyproline, tyrosine, hydroxylysine, methionine, cysteine, lysine, asparagine, or glutamine.  
     
     
         6 . The composition of  claim 4  wherein the glycosulfopeptide is combined with a polymer to form the compound.