Platelet glycoprotein v gene and uses

Abstract

This invention relates to the glycoprotein v gene. Specifically, this invention discloses the sequence and structure of the glycoprotein v gene and the amino acid sequence of the glycoprotein v polypeptide. In addition, the evolutionary relationship of the glycoprotein v gene with other glycoproteins is described and several uses of the isolated glycoprotein v gene are shown.

What is claimed is:  
     
         1 . An isolated DNA construct comprising a polynucleotide sequence encoding a glycoprotein V polypeptide.  
     
     
         2 . The DNA construct of  claim 1 , wherein the polynucleotide sequence encodes a polypeptide having an amino acid sequence as shown in FIG. 5B (SEQ.ID.NO. 2).  
     
     
         3 . The DNA construct of  claim 1 , wherein the polynucleotide sequence has a sequence as shown in FIG. 5A (SEQ.ID.NO. 1).  
     
     
         4 . The DNA construct of  claim 1 , wherein the polynucleotide sequence lacks introns.  
     
     
         5 . The DNA construct of  claim 1 , wherein the polynucleotide sequence encodes a full length glycoprotein V protein.  
     
     
         6 . The DNA construct of  claim 1 , further comprising a heterologous promoter operably linked to the polynucleotide sequence.  
     
     
         7 . The DNA construct of  claim 6 , wherein the promoter directs expression of the polynucleotide sequence in a eukaryote.  
     
     
         8 . The DNA construct of  claim 6 , wherein the promoter directs expression of the polynucleotide sequence in a prokaryote.  
     
     
         9 . A composition comprising a cell containing a DNA construct of  claim 1 .  
     
     
         10 . The composition of  claim 9 , wherein the cell is a eukaryote.  
     
     
         11 . The composition of  claim 9 , wherein the cell is a prokaryote.  
     
     
         12 . A pharmaceutical composition comprising a pharmaceutically acceptable carrier and the GPV polypeptide.  
     
     
         13 . The composition of  claim 12 , wherein the GPV polypeptide has a sequence as shown in FIG. 5B (SEQ.ID.NO. 2).  
     
     
         14 . An isolated, purified GPV polypeptide having a sequence as shown in FIG. 5B (SEQ.ID.NO. 2).  
     
     
         15 . An antibody capable of reacting with the polypeptide of  claim 14 .  
     
     
         16 . A method for inducing a degree of thrombocytopenia in a patient comprising the administration of the antibody of  claim 15 .  
     
     
         17 . The use of an isolated GPV promoter to direct expression of a polypeptide.  
     
     
         18 . A method for the detection of cells of megakaryoblastic lineage, comprising evaluating a sample for the presence or absence of a polynucleotide sequence as shown in FIG. 5A (SEQ.ID.NO. 1).  
     
     
         19 . A method for detecting thrombus formation in a subject, comprising the measurement of circulating GPVf1.  
     
     
         20 . An isolated nucleic acid capable of hybridizing to a nucleic acid having a sequence of FIG. 5A (SEQ.ID.NO. 1).