Human serine protease and serpin polypeptides

Abstract

The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.

What is claimed is:  
     
         1 . An isolated nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence at least 95% identical to a sequence selected from the group consisting of: 
 (a) residues 32 to 283 of SEQ ID NO: 2;    (b) residues 24 to 207 of SEQ ID NO: 4;    (c) residues 18 to 162 of SEQ ID NO: 6;    (d) residues 20 to 422 of SEQ ID NO: 8;    (e) residues 50 to 316 of SEQ ID NO: 10; and    (f) residues 20 to 76 of SEQ ID NO: 12.    
     
     
         2 . The isolated nucleic acid molecule of  claim 1  comprising the nucleotide sequence selected from the group consisting of: SEQ ID NO: 1 and SEQ ID NO: 3.  
     
     
         3 . The isolated nucleic acid molecule of  claim 1  comprising the nucleotide sequence selected from the group consisting of: SEQ ID NO: 5 and SEQ ID NO: 7.  
     
     
         4 . The isolated nucleic acid molecule of  claim 1  comprising the nucleotide sequence selected from the group consisting of: SEQ ID NO: 9 and SEQ ID NO: 11.  
     
     
         5 . The isolated nucleic acid molecule of  claim 1  comprising the nucleotide sequence shown as SEQ ID NO: 1.  
     
     
         6 . An isolated nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence selected from the group consisting of: 
 (a) at least 30 contiguous amino acid residues of SEQ ID NO: 2;    (b) at least 30 contiguous amino acid residues of SEQ ID NO: 4;    (c) at least 30 contiguous amino acid residues of SEQ ID NO: 6;    (d) at least 30 contiguous amino acid residues of SEQ ID NO: 8;    (e) at least 30 contiguous amino acid residues of SEQ ID NO: 10; and    (f) at least 30 contiguous amino acid residues of SEQ ID NO: 12.    
     
     
         7 . A vector comprising the isolated nucleic acid molecule of  claim 1 .  
     
     
         8 . A nucleic acid molecule comprising the nucleic acid molecule of  claim 1  operably associated with a heterologous regulatory element which controls gene expression.  
     
     
         9 . A host cell comprising the vector of  claim 7 .  
     
     
         10 . A host cell comprising the nucleic acid molecule of  claim 8 .  
     
     
         11 . An isolated polypeptide comprising an amino acid sequence at least 95% identical to a sequence selected from the group consisting of: 
 (a) residues 32 to 283 of SEQ ID NO: 2;    (b) residues 24 to 207 of SEQ ID NO: 4;    (c) residues 18 to 162 of SEQ ID NO: 6;    (d) residues 20 to 422 of SEQ ID NO: 8;    (e) residues 50 to 316 of SEQ ID NO: 10; and    (f) residues 20 to 76 of SEQ ID NO: 12.    
     
     
         12 . An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: 
 (a) at least 30 contiguous amino acid residues of SEQ ID NO: 2;    (b) at least 30 contiguous amino acid residues of SEQ ID NO: 4;    (c) at least 30 contiguous amino acid residues of SEQ ID NO: 6;    (d) at least 30 contiguous amino acid residues of SEQ ID NO: 8;    (e) at least 30 contiguous amino acid residues of SEQ ID NO: 10; and    (f) at least 30 contiguous amino acid residues of SEQ ID NO: 12.    
     
     
         13 . An isolated antibody that binds specifically to the isolated polypeptide of  claim 11 .  
     
     
         14 . A composition comprising the polypeptide of  claim 11 .  
     
     
         15 . A method of making an isolated polypeptide comprising: 
 (a) culturing the host cell of  claim 10  under conditions such that said polypeptide is expressed; and    (b) recovering said polypeptide.    
     
     
         16 . The polypeptide produced by the method of  claim 15 .  
     
     
         17 . A method for treating a medical condition, comprising administering to a patient a therapeutically effective amount of the polypeptide of  claim 11 .  
     
     
         18 . A method of diagnosing a pathological condition or a susceptibility to a pathological condition in a subject comprising: 
 (a) determining the presence or absence of a mutation in the polynucleotide of  claim 1;  and    (b) diagnosing a pathological condition or a susceptibility to a pathological condition based on the presence or absence of said mutation.    
     
     
         19 . A method of diagnosing a pathological condition or a susceptibility to a pathological condition in a subject comprising: 
 (a) determining the presence or amount of expression of the polypeptide of  claim 11  in a biological sample; and    (b) diagnosing a pathological condition or a susceptibility to a pathological condition based on the presence or amount of expression of the polypeptide.    
     
     
         20 . A method for identifying a binding partner to the polypeptide of  claim 11  comprising: 
 (a) contacting the polypeptide of  claim 11  with a binding partner; and  
 (b) determining whether the binding partner effects an activity of the polypeptide.