Additive solution for blood preservation

Abstract

There is provided compositions and methods for the storage of red blood cells. The compositions are additive solutions comprising adenine, dextrose, mannitol, NaH 2 PO 4 , and optionally NaCl and/or NH 4 Cl. Composition are preferably used with oxygen-depletion refrigerated storage of red blood cells and may optionally be employed with nutrient supplements extending the useful shelf life of stored blood.

What is claimed is:  
     
         1 . A method for the storage of red blood cells comprising: 
 mixing additive solution OFAS3 with packed red blood cells creating a suspension of red blood cells;    reducing the oxygen in said suspension of red blood cells to ≦10% of the level in whole blood by flushing with inert gas; and    storing said red blood cells under refrigeration at a temperature of about 4° C.    
     
     
         2 . A method according to  claim 1 , first comprising mixing whole blood with an anticoagulant solution, concentrating the red blood cells from said whole blood and separating the plasma thereby creating a mass of packed red blood cells.  
     
     
         3 . A method according to  claim 1 , further comprising storing said red blood cells under oxygen-depleted refrigeration.  
     
     
         4 . A method according to  claim 1 , further comprising adding a metabolic supplement at least once to said refrigerated red blood cells.  
     
     
         5 . A method according to  claim 4 , wherein said metabolic supplement comprises pyruvate, inosine, adenine and optionally dibasic sodium phosphate and/or monobasic sodium phosphate.  
     
     
         6 . An additive solution, OFAS3, for blood preservation comprising adenine, dextrose, mannitol, NaH 2 PO 4 , and optionally NaCl and/or NH 4 Cl.  
     
     
         7 . An additive solution according to  claim 6 , comprising about 0.5-4.0 mmole/liter of adenine, about 50-150 mmole/liter of dextrose, about 20-70 mmole/liter of mannitol, about 0-100 mmole/liter of NaCl, about 2-20 mmole/liter of NaH 2 PO 4 , and about 0-30 mmole/liter NH 4 Cl.  
     
     
         8 . An additive solution according to  claim 7 , having an adjusted pH from about 5.5-7.5.  
     
     
         9 . An additive solution according to  claim 7 , comprising about 2 mmole/liter adenine, about 110 mmole/liter dextrose, about 55 mmole/liter NaCl, and about 12 mmole/liter NaH 2 PO 4    
     
     
         10 . An additive solution according to  claim 9 , having an adjusted pH of about 6.5.