Nucleic acid molecules encoding CASPR/p190

Abstract

The 190 kDa Contactin ASsociated PRotein (CASPR/p190) is identified and is implicated as the bridge between contactin and intracellular second messenger systems for the signal caused by the binding of the carboxy anhydrase domain of RPTPβ to contactin and resulting in neurite growth, differentiation or survival. Mammalian CASPR/p190 cDNAs and proteins are described, including those from human and rat. In addition, particular domains of the proteins are characterized.

What is claimed is:  
     
         1 . An isolated nucleic acid molecule having a nucleotide sequence that: 
 (a) encodes the amino acid sequence SEQ ID NO:2;    (b) encodes the amino acid sequence SEQ ID NO:4; or    (c) is the complement of the nucleotide sequence of (a) or (b).    
     
     
         2 . An isolated nucleic acid molecule having a nucleotide sequence that hybridizes under highly stringent conditions to the nucleic acid molecule of  claim 1 , and encodes a naturally occurring p190 polypeptide.  
     
     
         3 . A nucleic acid molecule which comprises (a) a nucleotide sequence that encodes a polypeptide having the amino acid sequence shown in SEQ ID NO:2 from amino acid residues 40-168, 199-330, 362-486, 544-576, 582-739, 809-938, 961-985, 1031-1077, 1083-1218, 1282-1306, or 1328-1369; or (b) the complement of the nucleotide sequence of (a).  
     
     
         4 . A nucleic acid molecule which comprises (a) a nucleotide sequence that encodes a p190 polypeptide lacking at least one domain which has an amino acid sequence shown in SEQ ID NO:2 from amino acid residues 40-168, 199-330, 362-486, 544-576, 582-739, 809-938, 961-985, 1031-1077, 1083-1218, 1282-1306, or 1328-1369; or (b) the complement of the nucleotide sequence of (a).  
     
     
         5 . A recombinant vector containing the nucleic acid molecule of claims  1 ,  2 ,  3 , or  4 .  
     
     
         6 . The recombinant vector of  claim 5  wherein the nucleic acid molecule is operatively associated with an element that controls the expression of the nucleic acid molecule in a host cell.  
     
     
         7 . An engineered host cell containing the nucleic acid molecule of  claim 1 ,  2 ,  3 , or  4 .  
     
     
         8 . An engineered host cell containing the nucleic acid molecule of  claim 1 ,  2 ,  3 , or  4  operatively associated with an element that controls the expressing of the nucleic acid molecule by the engineered host cell.  
     
     
         9 . The engineered host cell of  claim 8  which is eukaryotic.  
     
     
         10 . The engineered host cell of  claim 8  which is prokaryotic.  
     
     
         11 . An isolated polypeptide comprising an amino acid sequence encoded by the nucleic acid molecule of  claim 1 ,  2 ,  3  or  4 .  
     
     
         12 . The isolated polypeptide of  claim 11 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4.  
     
     
         13 . A method for preparing a p190 polypeptide comprising: 
 (a) culturing a eukaryotic host cell which contains the nucleotide sequence of  claim 1 ,  2 ,  3 , or  4  operatively associated with an element that controls expression of the DNA sequence so that a p190 polypeptide is expressed by the host cell; and    (b) recovering the p190 polypeptide from the culture.    
     
     
         14 . A method for preparing a p190 polypeptide comprising: 
 (a) culturing a prokaryotic host cell which contains the nucleotide sequence of  claim 1 ,  2 ,  3 , or  4  operatively associated with an element that controls expression of the DNA sequence so that a p190 polypeptide is expressed by the host cell; and    (b) recovering the p190 polypeptide from the culture.    
     
     
         15 . The isolated nucleic acid molecule of  claim 1  wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:3.