Flower organ-specific gene and its promoter sequence

Abstract

This invention provides a flower organ-specific promoter enabling genetic engineering manipulations of pistil or lodicule. This invention further provides a chitinase expressed specifically in flower organ and its gene.

1 . A DNA fragment comprising the sequence from positions 114 to 1097, a part of said sequence or a sequence which is hybridizable with one of said sequences under conditions of moderate stringency, wherein said hybridizable sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 114 to 1097.  
     
     
         2 . A DNA fragment comprising the sequence from positions 114 to 1097 or a sequence which is hybridizable with said sequences under conditions of moderate stringency, wherein said hybridizable sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 114 to 1097.  
     
     
         3 . A DNA fragment comprising the sequence from positions 114 to 1097, a part of said sequence or a sequence having a homology of at least 80% to one of said sequences, wherein said part of the sequence and said homologous sequence encode a protein having a biological activity substantially the same as that encoded by the sequence from positions 114 to 1097.  
     
     
         4 . A DNA fragment comprising the sequence from positions 114 to 1097 or a sequence having a homology of at least 80% to said sequence, wherein said homologous sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 114 to 1097.  
     
     
         5 . A DNA fragment comprising the sequence from positions 114 to 1097, a part of said sequence or a sequence derived from one of said sequences by deletion, substitution, insertion or addition of one or more nucleotides, wherein said part of the sequence and said derived sequence encode a protein having a biological activity substantially the same as that encoded by the sequence form positions 114 to 1097.  
     
     
         6 . A DNA fragment comprising the sequence from positions 114 to 1097 or a sequence derived from said sequence by deletion, substitution, insertion or addition of one or more nucleotides, wherein said derived sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 114 to 1097.  
     
     
         7 . A peptide comprising the amino acid sequence of SEQ ID NO:2, a part of said amino acid sequence or an amino acid sequence having a homology of at least 60% to one of said amino acid sequences.  
     
     
         8 . A peptide comprising the amino acid sequence of SEQ ID NO:2 or an amino acid sequence having a homology of at least 60% to said amino acid sequence.  
     
     
         9 . A peptide comprising the amino acid sequence of SEQ ID NO:2, a part of said amino acid sequence or an amino acid sequence having a homology of at least 80% to one of said amino acid sequences.  
     
     
         10 . A peptide comprising the amino acid sequence of SEQ ID NO:2 or an amino acid sequence having a homology of at least 80% to said amino acid sequence.  
     
     
         11 . A peptide comprising the amino acid sequence of SEQ ID NO:2, a part of said amino acid sequence or an amino acid sequence derived from one of said amino acid sequences by deletion, substitution, insertion or addition of one or more amino acids.  
     
     
         12 . A peptide comprising the amino acid sequence of SEQ ID NO:2 or an amino acid sequence derived from said amino acid sequence by deletion, substitution, insertion or addition of one or more amino acids.  
     
     
         13 . A DNA fragment comprising the sequence from positions 174 to 1097, a part of said sequence or a sequence which is hybridizable with one of said sequences under conditions of moderate stringency, wherein said part of the sequence and said hybridizable sequence encode a protein having a biological activity substantially the same as that encoded by the sequence from positions 174 to 1097.  
     
     
         14 . A DNA fragment comprising the sequence from positions 174 to 1097, or a sequence which is hybridizable with said sequences under conditions of moderate stringency, wherein said hybridizable sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 174 to 1097.  
     
     
         15 . A DNA fragment comprising the sequence from positions 174 to 1097, a part of said sequence or a sequence having a homology of at least 80% to one of said sequences, wherein said part of the sequence and said homologous sequence encode a protein having a biological activity substantially the same as that encoded by the sequence from positions 174 to 1097.  
     
     
         16 . A DNA fragment comprising the sequence from positions 174 to 1097, or a sequence having a homology of at least 80% to said sequence, wherein said homologous sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 174 to 1097.  
     
     
         17 . A DNA fragment comprising the sequence from positions 174 to 1097, a part of said sequence or a sequence derived from one of said sequences by deletion, substitution, insertion or addition of one or more nucleotides, wherein said part of the sequence and said derived sequence encode a protein having a biological activity substantially the same as that encoded by the sequence from positions 174 to 1097.  
     
     
         18 . A DNA fragment comprising the sequence from positions 174 to 1097, or a sequence derived from said sequence by deletion, substitution, insertion or addition of one or more nucleotides, wherein said derived sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 174 to 1097.  
     
     
         19 . A DNA fragment comprising the sequence from positions 363 to 1097, a part of said sequence or a sequence which is hybridizable with one of said sequences under conditions of moderate stringency, wherein said part of the sequence and said hybridizable sequence encode a protein having a biological activity substantially the same as that encoded by the sequence from positions 363 to 1097.  
     
     
         20 . A DNA fragment comprising the sequence from positions 363 to 1097, or a sequence which is hybridizable with said sequences under conditions of moderate stringency, wherein said bybridizable sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 363 to 1097.  
     
     
         21 . A DNA fragment comprising the sequence from positions 363 to 1097, a part of said sequence or a sequence having a homology of at least 80% to one of said sequences, wherein said part of the sequence and said homologous sequence encode a protein having a biological activity substantially the same as that encoded by the sequence from positions 363 to 1097.  
     
     
         22 . A DNA fragment comprising the sequence from positions 363 to 1097, or a sequence having a homology of at least 80% to said sequence, wherein said homologous sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 363 to 1097.  
     
     
         23 . A DNA fragment comprising the sequence from positions 363 to 1097, a part of said sequence or a sequence derived from one of said sequences by deletion, substitution, insertion or addition of one or more nucleotides, wherein said part of the sequence and said derived sequence encode a protein having a biological activity substantially the same as that encoded by the sequence from positions 363 to 1097.  
     
     
         24 . A DNA fragment comprising the sequence from positions 363 to 1097, or a sequence derived from said sequence by deletion, substitution, insertion or addition of one or more nucleotides, wherein said derived sequence encodes a protein having a biological activity substantially the same as that encoded by the sequence from positions 363 to 1097.  
     
     
         25 . A peptide comprising the amino acid sequence from positions 21 to 328 in the sequence of SEQ ID NO:2, a part of said amino acid sequence or an amino acid sequence having a homology of at least 60% to one of said amino acid sequences.  
     
     
         26 . A peptide comprising the amino acid sequence from positions 21 to 328 in the sequence of SEQ ID NO:2 or an amino acid sequence having a homology of at least 60% to said amino acid sequence.  
     
     
         27 . A peptide comprising the amino acid sequence from positions 21 to 328 in the sequence of SEQ ID NO:2, a part of said amino acid sequence or an amino acid sequence having a homology of at least 80% to one of said amino acid sequences.  
     
     
         28 . A peptide comprising the amino acid sequence from positions 21 to 328 in the sequence of SEQ ID NO:2 or an amino acid sequence having a homology of at least 80% to said amino acid sequence.  
     
     
         29 . A peptide comprising the amino acid sequence from positions 21 to 328 in the sequence of SEQ ID NO:2, a part of said amino acid sequence or an amino acid sequence derived from one of said amino acid sequence by deletion, substitution, insertion or addition of one or more amino acids.  
     
     
         30 . A peptide comprising the amino acid sequence from positions 21 to 328 in the sequence of SEQ ID NO:2 or an amino acid sequence derived from said amino acid sequence by deletion, substitution, insertion or addition of one or more amino acids.  
     
     
         31 . A peptide comprising the amino acid sequence from positions 84 to 328 in the sequence of SEQ ID NO:2, a part of said amino acid sequence or an amino acid sequence having a homology of at least 60% to one of said amino acid sequences.  
     
     
         32 . A peptide comprising the amino acid sequence from positions 84 to 328 in the sequence of SEQ ID NO:2 or an amino acid sequence having a homology of at least 60% to said amino acid sequence.  
     
     
         33 . A peptide comprising the amino acid sequence from positions 84 to 328 in the sequence of SEQ ID NO:2, a part of said amino acid sequence or an amino acid sequence having a homology of at least 80% to one of said amino acid sequences.  
     
     
         34 . A peptide comprising the amino acid sequence from positions 84 to 328 in the sequence of SEQ ID NO:2 or an amino acid sequence having a homology of at least 80% to said amino acid sequence.  
     
     
         35 . A peptide comprising the amino acid sequence from positions 84 to 328 in the sequence of SEQ ID NO:2, a part of said amino acid sequence or an amino acid sequence derived from one of said amino acid sequences by deletion, substitution, insertion or addition of one or more amino acids.  
     
     
         36 . A peptide comprising the amino acid sequence from positions 84 to 328 in the sequence of SEQ ID NO:2 or an amino acid sequence derived from said amino acid sequence by deletion, substitution, insertion or addition of one or more amino acids.