Nucleic acid molecules encoding human kinase and phosphatase homologues and uses therefor

Abstract

The invention provides isolated nucleic acids molecules, designated Kinase and Phosphatase nucleic acid molecules, which encode novel protein kinase and protein phosphatase polypeptides. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing Kinase and Phosphatase nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a Kinase and Phosphatase gene has been introduced or disrupted. The invention still further provides isolated Kinase and Phosphatase proteins, fusion proteins, antigenic peptides and anti-Kinase and Phosphatase antibodies. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.

What is claimed:  
     
         1 . A method for detecting the presence of a Kinase or Phosphatase encoded by the nucleotide sequence shown in SEQ ID NOs: 1-14, in a sample comprising: 
 a) contacting the sample with a compound which selectively binds to the Kinase or Phosphatase; and    b) determining whether the compound binds to the Kinase or Phosphatase in the sample to thereby detect the presence of a Kinase or Phosphatase in the sample.    
     
     
         2 . The method of  claim 1 , wherein the compound which binds to the Kinase or Phosphatase is an antibody.  
     
     
         3 . A method for detecting the presence of a Kinase or Phosphatase nucleic acid molecule shown in SEQ ID NOs: 1-14 in a sample comprising: 
 a) contacting the sample with a nucleic acid probe or primer which selectively hybridizes to the nucleic acid molecule; and    b) determining whether the nucleic acid probe or primer binds to a nucleic acid molecule in the sample to thereby detect the presence of a Kinase or Phosphatase nucleic acid molecule in the sample.    
     
     
         4 . The method of  claim 3 , wherein the sample comprises mRNA molecules and is contacted with a nucleic acid probe.  
     
     
         5 . A method for identifying a compound which binds to a Kinase or Phosphatase encoded by the nucleotide sequence shown in SEQ ID NOs: 1-14 comprising: 
 a) contacting the Kinase or Phosphatase with a test compound under conditions suitable for binding; and    b) detecting binding of the test compound to the Kinase or Phosphatase.    
     
     
         6 . The method of  claim 5 , wherein said detection is by direct binding.  
     
     
         7 . The method of  claim 6 , wherein said direct binding is determined by an immunoprecipitation.  
     
     
         8 . The method of  claim 6 , wherein said direct binding is determined by a yeast two-hybrid assay.  
     
     
         9 . A method for modulating the activity of a Kinase or Phosphatase encoded by the nucleotide sequence shown in SEQ ID NOs: 1-14 comprising contacting the Kinase or Phosphatase with a compound which binds to the Kinase or Phosphatase in a sufficient concentration to modulate the activity of the Kinase or Phosphatase.  
     
     
         10 . A method for identifying a compound which modulates the activity of a Kinase or Phosphatase encoded by the nucleotide sequence shown in SEQ ID NOs: 1-14 comprising: 
 a) contacting a Kinase or Phosphatase with a test compound; and    b) determining the effect of the test compound on the activity of the Kinase or Phosphatase to thereby identify a compound which modulates the activity of the Kinase or Phosphatase.