DNA chip and method for producing the same

Abstract

A DNA chip includes a large number of minute spots formed by dripping sample solutions onto a base plate. The base plate is provided with positional deviation-correcting means for displacing the spots and automatically correcting any positional deviation of each of the spots. The positional deviation-correcting means includes any one of at least one projection, at least one recess and electric field-generating means for providing charged states on the base plate centered at a correct position for each of the spots supplied on the base plate.

1 . A DNA chip comprising a plurality of sample spots supplied on a base plate, wherein: 
 said base plate is provided with positional deviation-correcting means for displacing said spots and automatically correcting any positional deviation of each of said plurality of spots after said spots are supplied on said base plate,    wherein said positional deviation-correcting means comprises at least one recess positioned on said base plate, said at least one recess being centered at a correct position for each of said plurality of spots supplied on said base plate.    
     
     
         2 . A DNA chip comprising a plurality of sample spots supplied on a base plate, wherein: 
 said base is provided with positional deviation-correcting means for displacing said spots and automatically correcting any positional deviation of each of said plurality of spots after said spots are supplied on said base plate,    wherein said positional deviation-correcting means comprises electric field-generating means for providing at least one charged state positioned on said base plate, said at least one charged state being centered at a correct position for each of said plurality of spots supplied on said base plate, wherein said at least one charged state is opposite to at least one charged state of said plurality of spots supplied on said base plate.    
     
     
         3 . A method for producing a DNA chip comprising a plurality of sample spots supplied on a base plate, comprising the steps of: 
 providing a base plate having positional deviation-correcting means for displacing said spots and automatically correcting any positional deviation of each of said plurality of spots after said spots are supplied on said base plate; and    supplying said plurality of spots onto said base plate,    wherein said positional deviation-correcting means comprises at least one recess positioned on said base plate, said at least one recess being centered at a correct position for each of said plurality of spots supplied on said base plate.    
     
     
         4 . The method for producing said DNA chip according to  claim 3 , wherein said step of supplying said plurality of spots on said base plate is performed in accordance with an ink-jet system using a supply apparatus.  
     
     
         5 . The method for producing said DNA chip according to  claim 4 , wherein said supply apparatus is a dispenser comprising a plurality of arranged micropipettes each including a pouring port for pouring said sample solution from the outside, a cavity for pouring and charging said sample solution thereinto, and a discharge port for discharging said sample solution supplied on said base plate, said micropipette further including a piezoelectric/electrostrictive element disposed on at least one wall surface of said substrate forming said cavity to operate as a means for moving said sample solution through said cavity, the method further comprises the step of discharging mutually different types of said sample solutions from said discharge ports of said respective micropipettes.  
     
     
         6 . The method for producing said DNA chip according to  claim 5 , wherein said sample solution moves in a laminar flow within said cavity in said dispenser comprising said plurality of arranged micropipettes.  
     
     
         7 . A method for producing a DNA chip comprising a plurality of sample spots supplied on a base plate, comprising the steps of: 
 providing a base plate having positional deviation-correcting means for displacing said spots and automatically correcting any positional deviation of each of said plurality of spots after said spots are supplied on said base plate; and    supplying said plurality of spots onto said base plate,    wherein said positional deviation-correcting means comprises electric field-generating means for providing a charged state on at least a portion of said base plate, said electric field-generating means being centered at a correct position for each of said plurality of spots supplied on said base plate, and said charged state being opposite to a charged state of said plurality of spots supplied on said base plate.    
     
     
         8 . The method for producing said DNA chip according to  claim 7 , wherein said step of supplying said plurality of spots on said base plate is performed in accordance with an ink-jet system using a supply apparatus.  
     
     
         9 . The method for producing said DNA chip according to  claim 8 , wherein said supply apparatus is a dispenser comprising a plurality of arranged micropipettes each including a pouring port for pouring said sample solution from the outside, a cavity for pouring and charging said sample solution thereinto, and a discharge port for discharging said sample solution supplied on said base plate, said micropipette further including a piezoelectric/electrostrictive element disposed on at least one wall surface of said substrate forming said cavity to operate as a means for moving said sample solution through said cavity, the method further comprises the step of discharging mutually different types of said sample solutions from said discharge ports of said respective micropipettes.  
     
     
         10 . The method for producing said DNA chip according to  claim 9 , wherein said sample solution moves in a laminar flow within said cavity in said dispenser comprising said plurality of arranged micropipettes.