US2003104440A1PendingUtilityA1

Production and use of normalized DNA libraries

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Assignee: DIVERSA CORPPriority: Jun 18, 1996Filed: Sep 3, 2002Published: Jun 5, 2003
Est. expiryJun 18, 2016(expired)· nominal 20-yr term from priority
Inventors:Jay M. ShortEric J. Mathur
C12N 15/1093C12N 15/1096C12Q 1/6811
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Claims

Abstract

Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A process for forming a normalized genomic DNA library from an environmental sample, which comprises the steps of: 
 (a) isolating a genomic DNA population from the environmental sample;    (b) at least one of the steps selected from the group consisting of (i) amplyfying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and    (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.

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