Novel human protein tyrosine kinase

Abstract

The present invention provides a novel protein tyrosine kinase (HPTYK) and polynucleotides which identify and encode HPTYK. The invention also provides genetically engineered expression vectors and host cells comprising the nucleic acid sequences encoding HPTYK and a method for producing HPTYK. The invention also provides pharmaceutical compositions containing HPTYK or antagonists to HPTYK, and for the use of these compositions in the treatment of diseases associated with the expression of HPTYK. Additionally, the invention provides for the use of antisense molecules to polynucleotides encoding HPTYK for the treatment of diseases associated with the expression of HPTYK. The invention also provides diagnostic assays which utilize the polynucleotide, or fragments or the complement thereof, to hybridize to the genomic sequence or transcripts of polynucleotides encoding HPTYK or antibodies which specifically bind to HPTYK.

What is claimed is:  
     
         1 . A substantially purified human protein tyrosine kinase polypeptide comprising the amino acid sequence of SEQ ID NO:1 or fragments thereof.  
     
     
         2 . The isolated and purified polynucleotide sequence encoding the polypeptide of  claim 1 .  
     
     
         3 . The isolated and purified polynucleotide sequence of  claim 2  comprising the sequence of SEQ ID NO:2 or variants thereof.  
     
     
         4 . A polynucleotide sequence which is complementary to the sequence of SEQ ID NO:2 or variants thereof.  
     
     
         5 . A recombinant expression vector comprising the polynucleotide sequence of  claim 2 .  
     
     
         6 . A recombinant host cell comprising the expression vector of  claim 5 .  
     
     
         7 . A method for producing a polypeptide comprising the amino acid sequence shown in SEQ ID NO:l, the method comprising the steps 
 a) culturing the host cell of  claim 6  under conditions suitable for the expression of the polypeptide; and    b) recovering the polypeptide from the host cell culture.    
     
     
         8 . A pharmaceutical composition comprising a substantially purified human protein tyrosine kinase polypeptide having the amino acid sequence of SEQ ID NO: 1 or fragments thereof in conjunction with a suitable pharmaceutical carrier.  
     
     
         9 . A purified antibody which binds specifically to the polypeptide of  claim 1 .  
     
     
         10 . A purified antagonist which specifically regulates or modulates the activity of the polypeptide of  claim 1 .  
     
     
         11 . A pharmaceutical composition comprising a substantially purified antagonist of the polypeptide of  claim 1  in conjunction with a suitable pharmaceutical carrier.