Antibody against glyceraldehyde-3-phosphate dehydrogenase and use thereof

Abstract

A novel monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH), its use and a hybridoma strain NCK-J24 which produces the antibody. Since the monoclonal antibody produced by the hybridoma of the present invention recognizes GAPDH selectively and strongly, it can be used in screening and diagnosis of an apoptosis-concerning protein and prevention and/or treatment of neurodegenerative diseases (Alzheimer's disease, Parkinson's disease, Parkinson's syndrome, Huntington's disease, Machado-Joseph's disease, amyotrophic lateral sclerosis, Creutzfeldt-Jacob's disease and the like) and GAPDH-concerning diseases (motor dysfunction and the like caused by spinal cord injury, spinal cord compression, cerebral ischemia, peripheral nerve injury and the like).

1 . A monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase produced by a hybridoma strain NCK-J24 (FERM BP-6912).  
     
     
         2 . A hybridoma strain NCK-J24 (FERM BP-6912).  
     
     
         3 . A method for screening an apoptosis-regulating substance, comprising using the antibody of  claim 1 .  
     
     
         4 . A method for diagnosing an apoptosis-concerned disease, comprising using the antibody of  claim 1 .  
     
     
         5 . An agent for preventing and/or treating an apoptosis-concemed disease, comprising using the antibody of  claim 1.