Transcriptional regulator
Abstract
A database search using the sequence of transcriptional regulator “RING3” having a bromodomain identified an EST sequence that is highly homologous with RING3. Using primers prepared based on the EST sequence, a gene encoding a novel transcriptional regulator having a bromodomain has been successfully isolated from a human testis cDNA library by polymerase chain reactions. The results of analysis of the isolated gene revealed that this gene is expressed strongly in testis cells with a potent proliferative ability. The use of the above transcriptional regulator and its gene enables screening candidate compounds for factors interacting with the transcriptional regulator or drugs controlling the activity of the regulator.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . A substantially pure polypeptide comprising an amino acid sequence at least 60% identical to SEQ ID NO:1, wherein the polypeptide regulates transcription of a gene and comprises a bromodomain.
2 . The polypeptide of claim 1 , wherein the amino acid sequence is at least 70% identical to SEQ ID NO:1.
3 . The polypeptide of claim 1 , wherein the amino acid sequence is at least 80% identical to SEQ ID NO:1.
4 . The polypeptide of claim 1 , wherein the amino acid sequence is at least 90% identical to SEQ ID NO:1.
5 . A substantially pure polypeptide comprising SEQ ID NO:1.
6 . A substantially pure polypeptide comprising the amino acid sequence of SEQ ID NO:1, with up to 30 conservative amino acid substitutions, wherein the polypeptide regulates transcription of a gene and comprises a bromodomain.
7 . A substantially pure polypeptide encoded by a nucleic acid that hybridizes under high stringency conditions to a probe the sequence of which consists of SEQ ID NO:2, wherein the polypeptide regulates transcription of a gene and comprises a bromodomain.
8 . An isolated nucleic acid encoding the polypeptide of claim 1 .
9 . An isolated nucleic acid encoding the polypeptide of claim 5 .
10 . An isolated nucleic acid encoding the polypeptide of claim 6 .
11 . An isolated nucleic acid comprising a strand that hybridizes under high stringency conditions to a single stranded probe consisting of SEQ ID NO:2.
12 . The isolated nucleic acid of claim 11 , wherein the nucleic acid encodes a polypeptide that regulates transcription of a gene and comprises a bromodomain.
13 . The nucleic acid of claim 12 , wherein the polypeptide comprises SEQ ID NO:1.
14 . The nucleic acid of claim 11 , wherein the strand is at least 15 nucleotides in length.
15 . A vector comprising the nucleic acid of claim 8 .
16 . A vector comprising the nucleic acid of claim 9 .
17 . A vector comprising the nucleic acid of claim 10 .
18 . A vector comprising the nucleic acid of claim 11 .
19 . A vector comprising the nucleic acid of claim 12 .
20 . A cultured host cell comprising the nucleic acid of claim 8 .
21 . A cultured host cell comprising the nucleic acid of claim 9 .
22 . A cultured host cell comprising the nucleic acid of claim 10 .
23 . A cultured host cell comprising the nucleic acid of claim 11 .
24 . A cultured host cell comprising the nucleic acid of claim 12 .
25 . An antibody that specifically binds to the polypeptide of claim 1 .
26 . A method of preparing a polypeptide, the method comprising culturing the host cell of claim 20 , wherein the host cell expresses the polypeptide, and isolating the polypeptide from the host cell.
27 . A method of screening for a compound that binds to a polypeptide, the method comprising
providing a polypeptide comprising an amino acid sequence at least 60% identical to SEQ ID NO:1; contacting a test compound with the polypeptide; and determining whether the test compound has bound to the polypeptide.
28 . A compound which specifically binds to the polypeptide of claim 1.Join the waitlist — get patent alerts
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