Secreted protein HPEAD48

Abstract

The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.

What is claimed is:  
     
         1 . An isolated antibody or fragment thereof that specifically binds to a protein selected from the group consisting of: 
 (a) a protein consisting of amino acid residues 1 to 98 of SEQ ID NO:73;    (b) a protein consisting of amino acid residues 19 to 98 of SEQ ID NO:73;    (c) a protein consisting of a portion of SEQ ID NO:73, wherein said portion comprises at least 30 contiguous amino acid residues of SEQ ID NO:73; and    (d) a protein consisting of a portion of SEQ ID NO:73, wherein said portion comprises at least 50 contiguous amino acid residues of SEQ ID NO:73.    
     
     
         2 . The antibody or fragment thereof of  claim 1  that specifically binds protein (a).  
     
     
         3 . The antibody or fragment thereof of  claim 1  that specifically binds protein (b).  
     
     
         4 . The antibody or fragment thereof of  claim 1  that specifically binds protein (c).  
     
     
         5 . The antibody or fragment thereof of  claim 1  that specifically binds protein (d).  
     
     
         6 . The antibody or fragment thereof of  claim 3  that specifically binds protein (a).  
     
     
         7 . The antibody or fragment thereof of  claim 3 , wherein said protein bound by said antibody or fragment thereof is glycosylated.  
     
     
         8 . The antibody or fragment thereof of  claim 3  which is a human antibody.  
     
     
         9 . The antibody or fragment thereof of  claim 3  which is a polyclonal antibody.  
     
     
         10 . The antibody or fragment thereof of  claim 3  which is selected from the group consisting of: 
 (a) a chimeric antibody;  
 (b) a humanized antibody;  
 (c) a single chain antibody; and  
 (d) a Fab fragment.  
 
     
     
         11 . The antibody or fragment thereof of  claim 3  which is labeled.  
     
     
         12 . The antibody or fragment thereof of  claim 11 , wherein the label is selected from the group consisting of: 
 (a) an enzyme;    (b) a fluorescent label;    (c) a luminescent label; and    (d) a bioluminescent label.    
     
     
         13 . The antibody or fragment thereof of  claim 3 , wherein said antibody or fragment thereof specifically binds to said protein in a Western blot.  
     
     
         14 . The antibody or fragment thereof of  claim 3 , wherein said antibody or fragment thereof specifically binds to said protein in an ELISA.  
     
     
         15 . An isolated cell that produces the antibody or fragment thereof of  claim 3 .  
     
     
         16 . A hybridoma that produces the antibody or fragment thereof of  claim 3 .  
     
     
         17 . A method of detecting a protein in a biological sample comprising: 
 (a) contacting the biological sample with the antibody or fragment thereof of  claim 3;  and    (b) detecting the protein in the biological sample.    
     
     
         18 . The method of  claim 17  wherein the antibody or fragment thereof is a polyclonal antibody.  
     
     
         19 . An isolated antibody or fragment thereof obtained from an animal that has been immunized with a protein selected from the group consisting of: 
 (a) a protein comprising the amino acid sequence of amino acid residues 1 to 98 of SEQ ID NO:73;    (b) a protein comprising the amino acid sequence of amino acid residues 19 to 98 of SEQ ID NO:73;    (c) a protein comprising the amino acid sequence of at least 30 contiguous amino acid residues of SEQ ID NO:73; and    (d) a protein comprising the amino acid sequence of at least 50 contiguous amino acid residues of SEQ ID NO:73;    wherein said antibody or fragment thereof specifically binds to said amino acid sequence.    
     
     
         20 . The antibody or fragment thereof of  claim 19  obtained from an animal immunized with protein (a).  
     
     
         21 . The antibody or fragment thereof of  claim 19  obtained from an animal immunized with protein (b).  
     
     
         22 . The antibody or fragment thereof of  claim 19  obtained from an animal immunized with protein (c).  
     
     
         23 . The antibody or fragment thereof of  claim 19  obtained from an animal immunized with protein (d).  
     
     
         24 . The antibody or fragment thereof of  claim 21  which is a monoclonal antibody.  
     
     
         25 . The antibody or fragment thereof of  claim 21  which is selected from the group consisting of: 
 (a) a chimeric antibody;  
 (b) a polyclonal antibody;  
 (c) a humanized antibody;  
 (d) a single chain antibody; and  
 (e) a Fab fragment.  
 
     
     
         26 . An isolated monoclonal antibody or fragment thereof that specifically binds to a protein selected from the group consisting of: 
 (a) a protein consisting of amino acid residues 1 to 98 of SEQ ID NO:73;    (b) a protein consisting of amino acid residues 19 to 98 of SEQ ID NO:73;    (c) a protein consisting of a portion of SEQ ID NO:73, wherein said portion comprises at least 30 contiguous amino acid residues of SEQ ID NO:73; and    (d) a protein consisting of a portion of SEQ ID NO:73, wherein said portion comprises at least 50 contiguous amino acid residues of SEQ ID NO:73.    
     
     
         27 . The antibody or fragment thereof of  claim 26  that specifically binds protein (a).  
     
     
         28 . The antibody or fragment thereof of  claim 26  that specifically binds protein (b).  
     
     
         29 . The antibody or fragment thereof of  claim 26  that specifically binds protein (c).  
     
     
         30 . The antibody or fragment thereof of  claim 26  that specifically binds protein (d).  
     
     
         31 . The antibody or fragment thereof of  claim 28  that specifically binds protein (a).  
     
     
         32 . The antibody or fragment thereof of  claim 28 , wherein said protein bound by said antibody or fragment thereof is glycosylated.  
     
     
         33 . The antibody or fragment thereof of  claim 28  which is a human antibody.  
     
     
         34 . The antibody or fragment thereof of  claim 28  which is selected from the group consisting of: 
 (a) a chimeric antibody;  
 (b) a humanized antibody;  
 (c) a single chain antibody; and  
 (d) a Fab fragment.  
 
     
     
         35 . The antibody or fragment thereof of  claim 28  which is labeled.  
     
     
         36 . The antibody or fragment thereof of  claim 35 , wherein the label is selected from the group consisting of: 
 (a) an enzyme;    (b) a fluorescent label;    (c) a luminescent label; and    (d) a bioluminescent label.    
     
     
         37 . The antibody or fragment thereof of  claim 28 , wherein said antibody or fragment thereof specifically binds to said protein in a Western blot.  
     
     
         38 . The antibody or fragment thereof of  claim 28 , wherein said antibody or fragment thereof specifically binds to said protein in an ELISA.  
     
     
         39 . An isolated cell that produces the antibody or fragment thereof of  claim 28 .  
     
     
         40 . A hybridoma that produces the antibody or fragment thereof of  claim 28 .  
     
     
         41 . A method of detecting a protein in a biological sample comprising: 
 (a) contacting the biological sample with the antibody or fragment thereof of  claim 28;  and    (b) detecting the protein in the biological sample.    
     
     
         42 . An isolated antibody or fragment thereof that specifically binds to a protein selected from the group consisting of: 
 (a) a protein consisting of the full-length polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277;    (b) a protein consisting of the secreted form of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277;    (c) a protein consisting of a portion of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277, wherein said portion comprises at least 30 contiguous amino acid residues of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277; and    (d) a protein consisting of a portion of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277, wherein said portion comprises at least 50 contiguous amino acid residues of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277.    
     
     
         43 . The antibody or fragment thereof of  claim 42  that specifically binds protein (a).  
     
     
         44 . The antibody or fragment thereof of  claim 42  that specifically binds protein (b).  
     
     
         45 . The antibody or fragment thereof of  claim 42  that specifically binds protein (c).  
     
     
         46 . The antibody or fragment thereof of  claim 42  that specifically binds protein (d).  
     
     
         47 . The antibody or fragment thereof of  claim 44  that specifically binds protein (a).  
     
     
         48 . The antibody or fragment thereof of  claim 44 , wherein said protein bound by said antibody or fragment thereof is glycosylated.  
     
     
         49 . The antibody or fragment thereof of  claim 44  which is a human antibody.  
     
     
         50 . The antibody or fragment thereof of  claim 44  which is a polyclonal antibody.  
     
     
         51 . The antibody or fragment thereof of  claim 44  which is selected from the group consisting of: 
 (a) a chimeric antibody;  
 (b) a humanized antibody;  
 (c) a single chain antibody; and  
 (d) a Fab fragment.  
 
     
     
         52 . The antibody or fragment thereof of  claim 44  which is labeled.  
     
     
         53 . The antibody or fragment thereof of  claim 52 , wherein the label is selected from the group consisting of: 
 (a) an enzyme;    (b) a fluorescent label;    (c) a luminescent label; and    (d) a bioluminescent label.    
     
     
         54 . The antibody or fragment thereof of  claim 44 , wherein said antibody or fragment thereof specifically binds to said protein in a Western blot.  
     
     
         55 . The antibody or fragment thereof of  claim 44 , wherein said antibody or fragment thereof specifically binds to said protein in an ELISA.  
     
     
         56 . An isolated cell that produces the antibody or fragment thereof of  claim 44 .  
     
     
         57 . A hybridoma that produces the antibody or fragment thereof of  claim 44 .  
     
     
         58 . A method of detecting a protein in a biological sample comprising: 
 (a) contacting the biological sample with the antibody or fragment thereof of  claim 44;  and    (b) detecting the protein in the biological sample.    
     
     
         59 . The method of  claim 58 , wherein the antibody or fragment thereof is a polyclonal antibody.  
     
     
         60 . An isolated antibody or fragment thereof obtained from an animal that has been immunized with a protein selected from the group consisting of: 
 (a) a protein comprising the amino acid sequence of the full-length polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277;    (b) a protein comprising the amino acid sequence of the secreted form of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277;    (c) a protein comprising the amino acid sequence of at least 30 contiguous amino acid residues of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277; and    (d) a protein comprising the amino acid sequence of at least 50 contiguous amino acid residues the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277;    wherein said antibody or fragment thereof specifically binds to said amino acid sequence.    
     
     
         61 . The antibody or fragment thereof of  claim 60  obtained from an animal immunized with protein (a).  
     
     
         62 . The antibody or fragment thereof of  claim 60  obtained from an animal immunized with protein (b).  
     
     
         63 . The antibody or fragment thereof of  claim 60  obtained from an animal immunized with protein (c).  
     
     
         64 . The antibody or fragment thereof of  claim 60  obtained from an animal immunized with protein (d).  
     
     
         65 . The antibody or fragment thereof of  claim 62  which is a monoclonal antibody.  
     
     
         66 . The antibody or fragment thereof of  claim 62  which is selected from the group consisting of: 
 (a) a chimeric antibody;  
 (b) a polyclonal antibody;  
 (c) a humanized antibody;  
 (d) a single chain antibody; and  
 (e) a Fab fragment.  
 
     
     
         67 . An isolated monoclonal antibody or fragment thereof that specifically binds to a protein selected from the group consisting of: 
 (a) a protein consisting of the full-length polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277;    (b) a protein consisting of the secreted form of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277;    (c) a protein consisting of a portion of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277, wherein said portion comprises at least 30 contiguous amino acid residues of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277; and    (d) a protein consisting of a portion of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277, wherein said portion comprises at least 50 contiguous amino acid residues of the polypeptide encoded by the HPEAD48 cDNA contained in ATCC Deposit Number 209277.    
     
     
         68 . The antibody or fragment thereof of  claim 67  that specifically binds protein (a).  
     
     
         69 . The antibody or fragment thereof of  claim 67  that specifically binds protein (b).  
     
     
         70 . The antibody or fragment thereof of  claim 67  that specifically binds protein (c).  
     
     
         71 . The antibody or fragment thereof of  claim 67  that specifically binds protein (d).  
     
     
         72 . The antibody or fragment thereof of  claim 69  that specifically binds protein (a).  
     
     
         73 . The antibody or fragment thereof of  claim 69 , wherein said protein bound by said antibody or fragment thereof is glycosylated.  
     
     
         74 . The antibody or fragment thereof of  claim 69  which is a human antibody.  
     
     
         75 . The antibody or fragment thereof of  claim 69  which is selected from the group consisting of. (a ) a chime ric antibody; 
 (b) a humanized antibody;  
 (c) a single chain antibody; and  
 (d) a Fab fragment.  
 
     
     
         76 . The antibody or fragment thereof of  claim 69  which is labeled.  
     
     
         77 . The antibody or fragment thereof of  claim 76 , wherein the label is selected from the group consisting of: 
 (a) an enzyme;    (b) a fluorescent label;    (c) a luminescent label; and    (d) a bioluminescent label.    
     
     
         78 . The antibody or fragment thereof of  claim 69 , wherein said antibody or fragment thereof specifically binds to said protein in a Western blot.  
     
     
         79 . The antibody or fragment thereof of  claim 69 , wherein said antibody or fragment thereof specifically binds to said protein in an ELISA.  
     
     
         80 . An isolated cell that produces the antibody or fragment thereof of  claim 69 .  
     
     
         81 . A hybridoma that produces the antibody or fragment thereof of  claim 69 .  
     
     
         82 . A method of detecting a protein in a biological sample comprising: 
 (a) contacting the biological sample with the antibody or fragment thereof of  claim 69;  and    (b) detecting the protein in the biological sample.    
     
     
         83 . An isolated antibody or fragment thereof that specifically binds a HPEAD48 protein purified from a cell culture, wherein the cells in said cell culture comprise a polynucleotide encoding amino acids 1 to 98 of SEQ ID NO:73 operably associated with a regulatory sequence that controls gene expression.  
     
     
         84 . The antibody or fragment thereof of  claim 83  which is a monoclonal antibody.  
     
     
         85 . The antibody or fragment thereof of  claim 83  which is a human antibody.  
     
     
         86 . The antibody or fragment thereof of  claim 83  which is selected from the group consisting of: 
 (a) a chimeric antibody;  
 (b) a polyclonal antibody;  
 (c) a humanized antibody;  
 (d) a single chain antibody; and  
 (e) a Fab fragment.  
 
     
     
         87 . The antibody or fragment thereof of  claim 83 , wherein said antibody or fragment thereof specifically binds to said protein in a Western blot.  
     
     
         88 . The antibody or fragment thereof of  claim 83 , wherein said antibody or fragment thereof specifically binds to said protein in an ELISA.