Protein crystallization in microfluidic structures

Abstract

A device for promoting protein crystal growth (PCG) using microfluidic channels. A protein sample and a solvent solution are combined within a microfluidic channel having laminar flow characteristics which forms diffusion zones, providing for a well defined crystallization. Protein crystals can then be harvested from the device. The device is particularly suited for microgravity conditions.

What is claimed is:  
     
         1 ) A device for promoting protein crystallization growth from solution, comprising: 
 a body structure;    means located within said body structure for introduction of at least one solution containing protein and at least one solution containing a solvent; and    at least one microfluidic channel connected to said introduction means wherein said protein solution and said solvent solution interact to induce formation of protein crystals within said channel.    
     
     
         2 ) The device of  claim 1 , wherein said protein solution and said solvent solution flow laminarly in parallel contact within said microfluidic channel to establish a concentration gradient within said channel, allowing for protein crystallization.  
     
     
         3 ) The device of  claim 1 , wherein said protein solution introduction means and said solvent solution introduction means are each connected to said crystallization channel by a microfluidic channel.  
     
     
         4 ) The device of  claim 3 , wherein said protein microfluidic channel, said solvent microfluidic channel, and said crystallization channel form a T-Sensor structure.  
     
     
         5 ) The device of  claim 1 , further comprising a chamber coupled to said crystallization channel for harvesting said formed protein crystals.  
     
     
         6 ) The device of  claim 1 , further comprising fluid movement generating means coupled to said protein solution introduction means and said solvent solution introduction means for propelling said solutions through said crystallization channel.  
     
     
         7 ) The device of  claim 6 , wherein said fluid movement generating means comprises an air bellows.  
     
     
         8 ) The device of  claim 1 , further comprising a mixing means, coupled between said protein solution introduction means and said solvent introduction means interface and said crystallization channel for mixing said protein solution and said solvent solution completely to form a homogeneous mixture.  
     
     
         9 ) The device of  claim 8 , wherein said mixing means comprises a jet vortex mixer.  
     
     
         10 ) The device of  claim 9 , further comprising a solvent absorbing means coupled to said crystallization channel for absorbing solvent from said homogeneous mixture to increase the concentration of protein within said mixture, thereby inducing increased protein crystallization.  
     
     
         11 ) A device for promoting protein crystallization growth from solution, comprising: 
 a body structure;    means located within said body structure for introduction of at least one solution containing protein, at least one solution containing a solvent, and at least one solution containing a combination of protein and a buffer;    a microfluidic structure coupled to said protein solvent introduction means and said combination protein and buffer solution introduction means for flowing said solutions laminarly in parallel to remove irreversible protein aggregates from said combined solutions;    and at least one microfluidic channel connected to said solvent introduction means and the output of said microfluidic structure wherein said solvent solution and said combined solutions interact to induce formation of protein crystals within said channel.    
     
     
         12 ) The device of  claim 11 , further comprising a waste chamber coupled to said microfluidic structure to retain said irreversible protein aggregates.  
     
     
         13 ) The device of  claim 11 , further comprising a chamber coupled to said crystallization chamber for harvesting said formed protein crystals.  
     
     
         14 ) The device of  claim 11 , further comprising fluid movement generating means coupled to said protein solution introduction means, said solvent solution introduction means, and said combined protein and buffer solution introduction means for propelling said solutions through said crystallization channel.  
     
     
         15 ) The device of  claim 14 , wherein said fluid movement generating means comprises an air pump.  
     
     
         16 ) The device of  claim 11 , wherein said microfluidic structure comprises an H-filter.  
     
     
         17 ) The device of  claim 1 , wherein said body structure is constructed from plastic.