Regeneration of both plant tissues and transgenic plant tissues using a new plant hormone, 5-bromoindole-3-acetic acid

Abstract

The present invention describes the use of 5-bromoindole-3-acetic acid (5-B-IAA) as an auxin affecting plant cell growth. The invention relates to the use of 5-B-IAA compositions to affect growth in monocotyledonous as well as in dicotyledonous plants. The invention also describes the use of 5-B-IAA in plant growth affecting compositions for the regeneration of both plant tissues and transgenic plant tissues. Further, the invention provides plant growth affecting compositions comprising 5-B-IAA alone or in a mixture comprising one or more additional plant growth regulators, such as cytokinin, etc.

We claim:  
     
         1 . A composition for affecting the growth of plants comprising a growth affecting, effective amount of 5-bromoindole-3-acetic acid, or a salt, an ester or an amide derivative thereof.  
     
     
         2 . The composition of  claim 1  further comprising a growth affecting effective amount of one or more plant growth regulators.  
     
     
         3 . The composition of  claim 1  further comprising a growth affecting effective amount of a cytokinin.  
     
     
         4 . The composition of  claim 3  wherein said cytokinin is selected from the group consisting of benzylaminopurine (BAP), N 6  (Δ 2  Isopentenyl) adenine (2iP) and 6-furfurylaminopurine (kinetin).  
     
     
         5 . A method for affecting the growth of a plant which comprises applying to said plant, a growth affecting, effective amount of a composition comprising 5-bromoindole-3-acetic acid, or a salt, an ester or an amide derivative thereof.  
     
     
         6 . The method of  claim 5  wherein said composition further comprises one or more other plant growth regulators.  
     
     
         7 . The method of  claim 5  wherein said composition further comprises a cytokinin.  
     
     
         8 . A method for affecting the growth of a plant locus, plant cell, plant tissue, immature plant seed, or mature plant seed which comprises applying to said plant locus, pant cell, plant tissue, immature plant seed, or mature plant seed, a growth-affecting, effective amount of a composition comprising 5-bromoindole-3-acetic acid, or s salt, an ester or ana mide derivative thereof.  
     
     
         9 . The method of  claim 8  wherein said plant is a plant tissue culture.  
     
     
         10 . The method of  claim 8  wherein said plant is a monocotyledon.  
     
     
         11 . The method of  claim 10  wherein said plant is  Oryza sativa.    
     
     
         12 . The method of  claim 8  wherein said plant is a dicotyledon.  
     
     
         13 . The method of  claim 8  wherein said plant is a transgenic plant.  
     
     
         14 . The method of  claim 13  wherein said plant is a monocotyledon.  
     
     
         15 . The method of  claim 14  wherein said plant is  Oryza sativa.    
     
     
         16 . The method of  claim 13  wherein said plant is a docotyledon.  
     
     
         17 . The method of  claim 16  wherein said plant is  Arabidopsis thaliana.    
     
     
         18 . The method of  claim 16  wherein said plant is Nicotiana tabacum.  
     
     
         19 . A method for enhancing the growth of a plant comprising the step of growing said plant in the presence of a growth enhancing, effective amount of a composition comprising 5-bromoindole-3-acetic acid, or a salt, an ester or an amide derivative thereof, such that an improvement in a plant growth characteristic occurs in said plant in the presence of said composition when compared to a corresponding plant in the absence of said composition.  
     
     
         20 . The method of  claim 19  wherein said composition further comprises one or more plant growth hormone.  
     
     
         21 . The method of  claim 19  wherein said composition comprises a cytokinin.  
     
     
         22 . The method of  claim 19  wherein said plant is a monocotyledon or a dicotyledon.  
     
     
         23 . The method of  claim 19  wherein said plant is a transgenic plant.