Novel substance derived from basidiomycete culture, method for producing it and its use

Abstract

A novel substance that has the physiological activity of the aglycone of isoflavones and the physiological activity of the culture of the basidiomycetes with the physiological activities being synergistically enhanced, obtained by cultivating a basidiomycetes having a β-glucosidase activity in a medium containing an isoflavone, a method for producing it, and food, feedstuff and medicine containing it. The substance of the invention is improved in the physiological effect of the aglycone of isoflavones and the physiological effect of the culture of the basidiomycetes, and is not only useful as an anti-tumor agent but also is useful for therapy and/or prevention of osteoporosis and also as an immune enhancement agent.

1 . A substance having a physiological effect, comprising an aglycone of isoflavone and a cultivation product of basidiomycetes, obtained by cultivating basidiomycetes having a β-glucosidase activity in a medium in the presence of isoflavones-containing materials.  
     
     
         2 . A substance having a physiological effect, comprising an aglycone of isoflavone and a cultivation product of basidiomycetes, obtained by cultivating basidiomycetes having a β-glucosidase activity in a medium containing a material that contains an isoflavone and β-glucosidase.  
     
     
         3 . A substance according to  claim 1  or  2 , wherein the physiological activity of the aglycone of isoflavone and the physiological activity of the basidiomycetes are synergistically increased.  
     
     
         4 . A substance according to any one of  claims 1  to  3 , wherein the aglycone of isoflavone is genistein.  
     
     
         5 . A substance according to any one of  claims 1  to  4 , wherein the physiological effect is anti-tumor effect.  
     
     
         6 . A substance according to  claim 5 , wherein the anti-tumor effect is a tumor angiogenesis inhibition effect.  
     
     
         7 . A substance according to  claim 5 , wherein the anti-tumor effect is a tumor cell growth suppression effect.  
     
     
         8 . A substance according to  claim 7 , wherein the tumor cell growth suppression effect is tumor cell apoptosis induction effect.  
     
     
         9 . A substance according to any one of  claims 1  to  4 , wherein the material that contains an isoflavone is soybean seed, a processed product derived from soybean seed or arrowroot.  
     
     
         10 . A substance according to  claim 1  or  2 , wherein the basidiomycetes that have a β-glucosidase activity is  Ganoderma lucidum  or  Lentinus edodes.    
     
     
         11 . A method for producing a substance having a physiological effect, comprising the steps of cultivating basidiomycetes having a β-glucosidase activity in a medium that contains a material containing an isoflavone and collecting a component that contains an aglycone of isoflavone and a cultivation product of the basidiomycetes.  
     
     
         12 . A method producing a substance having a physiological effect according to  claim 11 , wherein the method comprises preliminarily cultivating the basidiomycetes to increase the β-glucosidase activity thereof, then introducing a material that contains an isoflavone in a medium, cultivating the basidiomycetes, and collecting a component that contains an aglycone of isoflavone and a cultivation product of the basidiomycetes.  
     
     
         13 . A method for producing a substance having a physiological effect, comprising the steps of cultivating basidiomycetes in a medium that contains a material containing an isoflavone and a β-glucosidase and collecting a component that contains an aglycone of an isoflavone and a cultivation product of basidiomycetes.  
     
     
         14 . A method for producing a substance having a physiological effect according to any one of  claims 11  to  13 , wherein the aglycone of isoflavone is genistein.  
     
     
         15 . A method according to any one of  claims 11  to  14 , wherein the physiological effect is an anti-tumor activity.  
     
     
         16 . A method according to  claim 15 , wherein the anti-tumor effect is a tumor angiogenesis inhibition effect.  
     
     
         17 . A method according to  claim 15 , wherein the anti-tumor effect is tumor cell growth inhibiting effect.  
     
     
         18 . A method according to  claim 17 , wherein the tumor cell growth inhibiting effect is tumor cell apoptosis induction effect.  
     
     
         19 . A method according to  claim 11  or  12 , wherein the material that contains an isoflavone is soybean seed, a processed product derived from soybean seed or arrowroot.  
     
     
         20 . A method according to  claims 11  to  13 , wherein the basidiomycetes having a β-glucosidase activity  Ganoderma lucidum  or  Lentinus edodes.    
     
     
         21 . A health-care food comprising a substance having a physiological effect according to any one of  claims 1  to  10 .  
     
     
         22 . A feedstuff composition comprising a substance having a physiological effect according to any one of  claims 1  to  10 .  
     
     
         23 . An anti-tumor agent comprising a substance having a physiological effect according to any one of  claims 1  to  10  as an active ingredient.