US2003064480A1PendingUtilityA1

Fusion proteins with immunoglobulin portions, the preparation and use thereof

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Priority: Jun 28, 1990Filed: Apr 30, 2002Published: Apr 3, 2003
Est. expiryJun 28, 2010(expired)· nominal 20-yr term from priority
Inventors:Leander LaufferGerd ZettlmeisslPatricia OquendoBrian Seed
C07K 2319/00C07K 14/745C07K 14/7155C07K 16/00C07K 14/505A61K 38/00
45
PatentIndex Score
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Claims

Abstract

The invention relates to genetically engineered soluble fusion proteins composed of human proteins not belonging to the immunoglobulin family, or of parts thereof, and of various portions of the constant region of immunoglobulin molecules. The functional properties of the two fusion partners are surprisingly retained in the fusion protein.

Claims

exact text as granted — not AI-modified
1 . A soluble fusion protein composed of human proteins not belonging to the immunoglobulin family, or of parts thereof, and of various portions of immunoglobulin molecules of all subclasses.  
     
     
         2 . A fusion protein as claimed in  claim 1 , wherein the immunoglobulin portion is the constant part of the heavy chain of human IgG.  
     
     
         3 . A fusion protein as claimed in  claim 2 , wherein the immunoglobulin portion is the constant part of the heavy chain of human IgG1 or a protein A-binding fragment thereof.  
     
     
         4 . A fusion protein as claimed in  claim 2  or  claim 3 , wherein the fusion takes place at the hinge region.  
     
     
         5 . A fusion protein as claimed in claims  1 - 4 , wherein the protein fused to immunoglobulin is the extra-cellular portion of a membrane protein or parts thereof.  
     
     
         6 . A fusion protein as claimed in claims  1 - 4 , wherein the protein fused to immunoglobulin is the extracellular portion of thromboplastin or parts thereof.  
     
     
         7 . A fusion protein as claimed in claims  1 - 4 , wherein the protein fused to immunoglobulin is the extracellular portion of CD28 or parts thereof.  
     
     
         8 . A fusion protein as claimed in claims  1 - 4 , wherein the protein fused to immunoglobulin is the extracellular portion of a cytokine receptor or growth factor receptor or parts thereof.  
     
     
         9 . A fusion protein as claimed in  claim 8 , wherein the protein fused to immunoglobulin is the extracellular portion of IL-4 receptor or parts thereof.  
     
     
         10 . A fusion protein as claimed in  claim 8 , wherein the protein fused to immunoglobulin is the extracellular portion of IL-7 receptor or parts thereof.  
     
     
         11 . A fusion protein as claimed in  claim 8 , wherein the protein fused to immunoglobulin is the extracellular portion of tumor necrosis factor receptor or parts thereof.  
     
     
         12 . A fusion protein as claimed in  claim 8 , wherein the protein fused to immunoglobulin is the extracellular portion of G-CSF receptor or parts thereof.  
     
     
         13 . A fusion protein as claimed in  claim 8 , wherein the protein fused to immunoglobulin is the extracellular portion of GM-CSF receptor or parts thereof.  
     
     
         14 . A fusion protein as claimed in  claim 8 , wherein the protein fused to immunoglobulin is the extracellular portion of erythropoietin receptor or parts thereof.  
     
     
         15 . A fusion protein as claimed in claims  1 - 4 , wherein the protein fused to immunoglobulin is a non-membrane-bound soluble protein or parts thereof.  
     
     
         16 . A fusion protein as claimed in  claim 15 , wherein the protein fused to immunoglobulin is a cytokine- or growth factor or parts thereof.  
     
     
         17 . A fusion protein as claimed in  claim 16 , wherein the protein fused to immunoglobulin is erythropoietin or parts thereof.  
     
     
         18 . A fusion protein as claimed in  claim 16 , wherein the protein fused to immunoglobulin is GM-CSF or G-CSF or parts thereof.  
     
     
         19 . A fusion protein as claimed in  claim 16 , wherein the protein fused to immunoglobulin is interleukins IL-1 to IL-8 or parts thereof.  
     
     
         20 . A process for preparing fusion proteins as claimed in any of claims  1 - 19 , which comprises introducing the DNA coding for these constructs into a mammalian cell expression system and, after expression, purifying the produced fusion protein by affinity chromatography via the immunoglobulin portion.  
     
     
         21 . The use of the fusion proteins as claimed in any of claims  1 - 19  for diagnosis.  
     
     
         22 . The use of the fusion proteins as claimed in any of claims  1 - 19  for therapy.

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