Transaminases and aminotranferases

Abstract

Thermostable transaminase and aminotransferase enzymes derived from various ammonifex, aquifex and pyrobaculum organisms are disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the pharmaceutical, agricultural and other industries.

We claim:  
     
         1 . An isolated polynucleotide having at least 70% identity to a polynucleotide encoding an enzyme having aminotransferase and/or transaminase activity, wherein said polynucleotide is amplified using an oligonucleotide primer pair selected from:  
       
         
           
                 
                 
               
                     
                 
                   5′ CCGAGAATTCATTAAAGAGGAGAAATTAACTATGATTGAAGACCCTATGGAC 
                   (SEQ. ID NO:1) 
                 
                   and 
                 
                   3′ CGAAGATCTTTAGCACTTCTCTCAGGTTC; 
                   (SEQ. ID NO:2) 
                 
                     
                 
                   5′ CCGAGAATTCATTAAAGAGGAGAAATTAACTATGGACAGGCTTGAAAAAGTA 
                   (SEQ ID NO:3) 
                 
                   and 
                 
                   3′ CGGAAGATCTTCAGCTAAGCTTCTCTAAGAA; 
                   (SEQ ID NO:4) 
                 
                     
                 
                   5′ CCGACAATTGATTAAAGAGGAGAAATTAACTATGTGGGAATTAGACCCTAAA 
                   (SEQ ID NO:5) 
                 
                   and 
                 
                   3′ CGGAGGATCCCTACACCTCTTTTTCAAGCT; 
                   (SEQ ID NO:6) 
                 
                     
                 
                   5′ CCGACAATTGATTAAAGAGGAGAAATTAACTATGACATACTTAATGAACAAT 
                   (SEQ ID NO:7) 
                 
                   and 
                 
                   3′ CGGAAGATCTTTATGAGAAGTCCCTTTCAAG; 
                   (SEQ ID NO:8) 
                 
                     
                 
                   5′ CCGAGAATTCATTAAAGAGGAGAAATTAACTATGCGGAAACTGGCCGAGCGG 
                   (SEQ ID NO:9) 
                 
                   and 
                 
                   3′ CGGAGGATCCTTAAAGTGCCGCTTCGATCAA; 
                   (SEQ ID NO:10) 
                 
                     
                 
                   5′ CCGACAATTGATTAAAGAGGAGAAATTAACTATGTGCGGGATAGTCGGATAC 
                   (SEQ ID NO:11) 
                 
                   and 
                 
                   3′ CGGAAGATCTTTATTCCACCGTGACCGTTTT; 
                   (SEQ ID NO:12) 
                 
                     
                 
                   5′ CCGACAATTGATTAAAGAGGAGAAATTAACTATGATACCCCAGAGGATTAAG 
                   (SEQ ID NO:13) 
                 
                   and 
                 
                   3′ CGGAAGATCTTTAAAGAGAGCTTGAAAGGGA; 
                   (SEQ ID NO:14) 
                 
                     
                 
                   5′ CCGAGAATTCATTAAAGAGGAGAAATTAACIATGAAGCCGTACGCTAAATAT 
                   (SEQ ID NO:15) 
                 
                   and 
                 
                   3′ CGGAAGATCTCTAATACACAGGAGTGATCCA; 
                   (SEQ ID NO:16) 
                 
                     
                 
                   5′ CCGAGAATTCATTAGAGGAGAAATTAACTATGGCAGTCAAAGTGCGGCCT 
                   (SEQ ID NO:33) 
                 
                   and 
                 
                   3′ -CGGAGGATCCTTATCCAAAGCTTCCAGGAAG; 
                   (SEQ ID NO:34) 
                 
                   or 
                 
                     
                 
                   5′ CCGAGAATTCATTAAAGAGGAGAAATTAACTATGAGAAAAGGACTTGCAAGT 
                   (SEQ ID NO:37) 
                 
                   and 
                 
                   3′ CGGAGGATCCTTAGATCTCTTCAAGGGCTTT. 
                   (SEQ ID NO:38)