Device for sequential discharge of flowable reagents

Abstract

The invention relates to a device in which at least two liquid or semi-liquid reagents can be stored separately. When used, the inventive device enables precise, chronologically defined, sequential reagent discharge in a reaction chamber by means of a linear movement without any prior mixing of said reagents.

1 . A device for sequentially discharging flowable reagents, comprising: 
 a receptacle comprising a receiving chamber with two opposite ends, the receptacle having a relief opening at the first end of the receiving chamber,    a pressure generating unit adapted to be positioned or arranged at the receptacle at the second end of the receiving chamber, and adapted to build pressure in the receiving chamber,    a reagent column contained in the receiving chamber, comprising at least two reagent volumes separated by a respective partition element, each partition element abutting the inside of the receptacle in a sealing manner and being slidable into the receiving chamber towards the first end thereof,    an outlet opening arranged at a branch between the first and the second end of the receiving chamber, the distance between the outlet opening and the relief opening being at least as great as the thickness of the first partition element between the outlet opening and the second end of the receiving chamber,    a holding force generating unit arranged in the region between the outlet opening and the relief opening in the receiving chamber, for generating a holding force acting at least on the partition elements within this region of the receiving chamber, which is great enough to cause a high flow resistance to the reagents in this region that is higher than the resistance occurring when the reagents flow out from the outlet opening.    
     
     
         2 . The device of  claim 1 , wherein the pressure generating unit comprises a plunger abutting the inside of the receptacle in a sealing manner and being slidable towards the first end of the receiving chamber.  
     
     
         3 . The device of  claim 1  or  2 , wherein an outlet channel is provided extending outside the receiving chamber and being connected to the receiving chamber through the outlet opening.  
     
     
         4 . The device of  claim 1  or  2 , wherein an outlet tubule having an outlet channel is provided, which extends in the direction of movement of the at least one partition element and extends from outside the receiving chamber into the same, and whose end located in the receiving chamber forms the outlet opening of the receiving chamber, and wherein the at least one partition element can be pierced by the outlet tubule.  
     
     
         5 . The device of  claim 2  or  3 , wherein the relief opening is communicated with the outlet channel.  
     
     
         6 . The device of one of  claims 1  to  5 , wherein the holding force generating unit is a unit increasing the friction of at least the lowermost partition element in the area of the outlet opening and the relief opening as compared to the remainder of the receiving chamber.  
     
     
         7 . The device of  claim 6 , wherein the holding force generating unit is a constriction in the portion of the receiving chamber between the outlet opening and the relief opening.  
     
     
         8 . The device of  claim 6 , wherein the holding force generating unit is an enlargement of the lowermost partition element making the same wider than the other partition elements.  
     
     
         9 . The device of  claim 6 , wherein the increase in friction is obtained by a corresponding structure of the contacting surfaces of the lowermost partition element and the receiving chamber.  
     
     
         10 . The device of one of  claims 1  to  5 , wherein the holding force generating unit is a spring whose resilience acts opposite to the direction of movement of the at least one partition element when the pressure generating unit acts thereupon.  
     
     
         11 . A device for sequentially discharging flowable reagents, comprising: 
 a capillary comprising a receiving chamber with a first and an opposite second open end,    a pressure generating unit adapted to be positioned or arranged at the second end of the capillary, and adapted to build pressure in the receiving chamber,    a reagent column contained in the receiving chamber, comprising at least two reagent volumes separated by a gas volume or a volume with a hydrophobic liquid inert to the reagents and substantially immiscible with these,    an outlet opening arranged in the capillary between the two open ends and comprising a hydrophilic membrane,    a closure member slidable in the capillary and, prior to being subjected to the effect of the pressure generating unit, located between the second end of the capillary and the outlet opening, and adapted to be slidably moved past the outlet opening towards the first end of the capillary due to the pressure built up by the pressure generating unit, and    a holding force generating unit arranged in the region between the outlet opening and the first end of the capillary, for generating a holding force acting on the closure member, which is great enough to cause a high flow resistance to the reagents in this region of the capillary that is higher than the resistance occurring when the reagents flow out from the outlet opening and the membrane.