Process for preparing porous collagen matrix from connective tissue

Abstract

The subject invention provides a process for preparing a porous collagen matrix from connective tissue, which comprises treating connective tissue with a solution of hydrogen peroxide and/or an acidic solution so as to obtain a porous collagen matrix.

We claim:  
     
         1 . A process for preparing a porous collagen matrix from connective tissue, said process comprising treating connective tissue with a solution of hydrogen peroxide effective for preparing the porous collagen matrix.  
     
     
         2 . The process according to  claim 1 , wherein the solution of hydrogen peroxide has a concentration between 0.1 and 10%.  
     
     
         3 . The process according to  claim 1 , wherein the connective tissue is treated at a temperature ranging from 4 to 60.  
     
     
         4 . The process according to  claim 1 , the process further comprising steps of freezing the connective tissue treated with a solution of hydrogen peroxide, wherein the step of freezing is carried out at a proper temperature with a proper temperature reduction rate, and lyophilizing the connective tissue effective for preparing the porous collagen matrix.  
     
     
         5 . A process for preparing a porous collagen matrix from connective tissue, said process comprising treating connective tissue with an acidic solution effective for preparing the porous collagen matrix.  
     
     
         6 . The process according to  claim 5 , wherein the acidic solution is selected from a group consisting of peracetic acid, hydrochloric acid, oxalic acid, acetic acid and sulfuric acid.  
     
     
         7 . The process according to  claim 5 , wherein the acidic solution has a concentration of 0.01 to 4M.  
     
     
         8 . The process according to  claim 5 , wherein the acidic solution comprises at least a salt with a proper concentration.  
     
     
         9 . The process according to  claim 8 , wherein the salt has a proper concentration of 0.01 to 4M of sodium chloride.  
     
     
         10 . The process according to  claim 5 , the process further comprising steps of freezing the connective tissue treated with an acidic solution, wherein the step of freezing is carried out at a proper temperature with a proper temperature reduction rate, and lyophilizing the connective tissue effective for preparing the porous collagen matrix.  
     
     
         11 . A process for preparing a porous collagen matrix from connective tissue, the process comprising steps of treating connective tissue with an acidic solution followed by a solution of hydrogen peroxide, freezing said connective tissue at a proper temperature under a proper temperature reduction rate, and lyophilizing said connective tissue effective for preparing the porous collagen matrix.  
     
     
         12 . The process according to  claim 11 , wherein the solution of hydrogen peroxide has a concentration between 0.1 and 10%.  
     
     
         13 . The process according to  claim 11 , wherein the connective tissue is treated at a temperature ranging from 4 to 60.  
     
     
         14 . The process according to  claim 11 , wherein the acidic solution is selected from a group consisting of peracetic acid, hydrochloric acid, oxalic acid, acetic acid and sulfuric acid.  
     
     
         15 . The process according to  claim 11 , wherein the treatment with an acidic solution is divided into two steps, a first step comprising treating the connective tissue with an acetic acid solution of 0.1 to 6M and a second step comprising treating the connective tissue with an acetic acid solution of 0.01 to 2M.  
     
     
         16 . The process according to  claim 15 , wherein the acetic acid solution of 0.01 to 2M comprises at least a salt.  
     
     
         17 . The process according to  claim 16 , wherein the salt comprises sodium chloride of 0.01 to 4M.  
     
     
         18 . The process according to  claim 11 , the process further comprising a step of treating the connective tissue with a solution containing SDS (sodium dodecyl sulphate).  
     
     
         19 . The process according to  claim 18 , wherein the solution contains 0.01 to 10% of SDS.  
     
     
         20 . The process according to  claim 19 , wherein the solution contains a chelating agent.  
     
     
         21 . The process according to  claim 20 , wherein the chelating agent is EDTA (ethylene diamine tetra-acetic acid) with a concentration of 0.01 to 100 mM.  
     
     
         22 . A porous collagen matrix prepared by the process according to  claim 1 .  
     
     
         23 . A porous collagen matrix prepared by the process according to  claim 4 .  
     
     
         24 . A porous collagen matrix prepared by the process according to  claim 5 .  
     
     
         25 . A porous collagen matrix prepared by the process according to  claim 7 .  
     
     
         26 . A porous collagen matrix prepared by the process according to  claim 9 .  
     
     
         27 . A porous collagen matrix prepared by the process according to  claim 10 .  
     
     
         28 . A porous collagen matrix prepared by the process according to  claim 11 .  
     
     
         29 . A porous collagen matrix prepared by the process according to  claim 15 .  
     
     
         30 . A porous collagen matrix prepared by the process according to  claim 17 .  
     
     
         31 . A porous collagen matrix prepared by the process according to  claim 20 .  
     
     
         32 . A porous collagen matrix prepared by the process according to  claim 22.