Human 2-19 protein homologue, z219a

Abstract

The present invention relates to polynucleotide and polypeptide molecules for z219a, a novel member of the human 2-19 protein family. The polypeptides, and polynucleotides encoding them, may be used for detecting human chromosomal abnormalities. The present invention also includes antibodies to the z219a polypeptides.

What is claimed is:  
     
         1 . An isolated polynucleotide that encodes a polypeptide comprising a sequence of amino acid residues that is at least 90% identical to an amino acid sequence selected from the group consisting of: 
 (a) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 26 (Tyr), to amino acid number 235 (Ser); and    (b) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 1 (Met), to amino acid number 235 (Ser).    
     
     
         2 . An isolated polynucleotide molecule selected from the group consisting of: 
 (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 194 to nucleotide 823;    (b) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 119 to nucleotide 823; and    (c) polynucleotide molecules complementary to (a) or (b).    
     
     
         3 . An isolated polynucleotide sequence according to  claim 1 , wherein the polynucleotide comprises nucleotide 1 to nucleotide 705 of SEQ ID NO:8.  
     
     
         4 . An isolated polynucleotide according to  claim 1 , wherein the polypeptide consists of a sequence of amino acid residues that is at least 90% identical to an amino acid sequence as shown in SEQ ID NO:2 from amino acid number 26 (Tyr), to amino acid number 235 (Ser).  
     
     
         5 . An isolated polynucleotide according to  claim 4 , wherein the z219a polypeptide consists of a sequence of amino acid residues as shown in SEQ ID NO:2 from amino acid number 26 (Tyr), to amino acid number 235 (Ser).  
     
     
         6 . The isolated polynucleotide molecule of  claim 1 , wherein the polynucleotide encodes a polypeptide that contains motifs 1, 2, 3, 4 and 5 spaced apart from N-terminus to C-terminus in a configuration M1-{25-26}-M2-{15}-M3-{11}-M4-{34-36}-M5.  
     
     
         7 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment encoding a z219a polypeptide that is at least 90% identical to an amino acid sequence as shown in SEQ ID NO:2 from amino acid number 26 (Tyr), to amino acid number 235 (Ser); and    a transcription terminator,    wherein the promoter is operably linked to the DNA segment, and the DNA segment is operably linked to the transcription terminator.    
     
     
         8 . An expression vector according to  claim 7 , further comprising a secretory signal sequence operably linked to the DNA segment.  
     
     
         9 . A cultured cell into which has been introduced an expression vector according to  claim 7 , wherein the cell expresses the polypeptide encoded by the DNA segment.  
     
     
         10 . A DNA construct encoding a fusion protein, the DNA construct comprising: 
 a first DNA segment encoding a polypeptide that is at least 90% identical to a sequence of amino acid residues 1 (Met) through 25 (Gly) of SEQ ID NO:2; and    second DNA segment encoding an additional polypeptide,    wherein the first and second DNA segments are connected in-frame; and    encode the fusion protein.    
     
     
         11 . An isolated polypeptide comprising a sequence of amino acid residues that is at least 90% identical to an amino acid sequence selected from the group consisting of: 
 (a) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:2 from amino acid number 26 (Tyr) to amino acid number 235 (Ser) of SEQ ID NO:2; and    (b) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:2 from amino acid residue number 1 (Met) to amino acid residue number 235 (Ser).    
     
     
         12 . An isolated polypeptide according to  claim 11 , wherein the polypeptide consists of a sequence of amino acid residues that is at least 90% identical to an amino acid sequence as shown in SEQ ID NO:2 from amino acid number 26 (Tyr) to amino acid number 235 (Ser).  
     
     
         13 . An isolated polypeptide according to  claim 12 , wherein the sequence of amino acid residues is as shown in SEQ ID NO:2 from amino acid number 26 (Tyr) to amino acid number 235 (Ser).  
     
     
         14 . The isolated polypeptide of  claim 11 , wherein the polypeptide molecule encodes motifs 1, 2, 3, 4 and 5 spaced apart from N-terminus to C-terminus in a configuration M1-{25-26}-M2-{15}-M3-{11}-M4-{34-36}-M5.  
     
     
         15 . A method of producing a z219a polypeptide comprising: 
 culturing a cell according to  claim 9;  and    isolating the z219a polypeptide produced by the cell.    
     
     
         16 . A method of producing an antibody to z219a polypeptide comprising: 
 inoculating an animal with a polypeptide selected from the group consisting of: 
 (a) a polypeptide consisting of 9 to 210 amino acids, wherein the polypeptide is at least 90% identical to a contiguous sequence of amino acids in SEQ ID NO:2 from amino acid number 26 (Tyr) to amino acid number 235 (Ser);  
 (b) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid number 26 (Tyr) to amino acid number 235 (Ser);  
 (c) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid number 59 (Arg) to amino acid number 133 (Asp);  
 (d) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid number 135 (Ser) to amino acid number 212 (Ala);  
 (e) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid 215 (Asn) to amino acid number 231 (Pro); and  
   wherein the polypeptide elicits an immune response in the animal to produce the antibody; and    isolating the antibody from the animal.    
     
     
         17 . An antibody produced by the method of  claim 16 , which specifically binds to a z219a polypeptide.  
     
     
         18 . The antibody of  claim 17 , wherein the antibody is a monoclonal antibody.  
     
     
         19 . An antibody which specifically binds to a polypeptide of  claim 11 .  
     
     
         20 . A method of detecting, in a test sample, the presence of an antagonist of z219a protein activity, comprising: 
 transfecting a z219a-responsive cell, with a reporter gene construct that is responsive to a z219a-stimulated cellular pathway; and    producing a z219a polypeptide by the method of  claim 15;  and    adding the z219a polypeptide to the cell, in the presence and absence of a test sample; and    comparing levels of response to the z219a polypeptide, in the presence and absence of the test sample, by a biological or biochemical assay; and    determining from the comparison, the presence of the antagonist of z219a activity in the test sample.    
     
     
         21 . A method of detecting, in a test sample, the presence of an agonist of z219a protein activity, comprising: 
 transfecting a z219a-responsive cell, with a reporter gene construct that is responsive to a z219a-stimulated cellular pathway; and    adding a test sample; and    comparing levels of response in the presence and absence of the test sample, by a biological or biochemical assay; and    determining from the comparison, the presence of the agonist of z219a activity in the test sample.