Apparatus for microinjection of sample into amphibian oocytes

Abstract

An apparatus for microinjection of samples into amphibian oocytes incorporates a tray for holding a plurality of the amphibian oocytes, an injection needle for injecting a sample into the amphibian oocytes, a driving means for moving a relative position between the tray and the injection needle and a controlling device for controlling the movement by inputting a depth of the injection needle for the tray or the amphibian oocytes in the injection of the sample, and injecting the sample into the amphibian oocytes at the given depth. According to the present invention, the sample can be injected into the amphibian oocyte with constant depth precisely , and quality of the oocytes and the positional site of needle injection can be recorded as information.

What is claimed is:  
     
         1 . A method for selling or assigning a plurality of amphibian oocytes, comprising selling or assigning a plurality of amphibian oocytes as a set, into which a sample is injected under a substantially same condition in injection depth.  
     
     
         2 . The method for selling or assigning a plurality of amphibian oocytes according to  claim 1 , wherein an information on the injection of said sample into said plurality of said amphibian oocytes is attached to the set.  
     
     
         3 . The method for selling or assigning a plurality of amphibian oocytes according to  claim 1 , wherein said information is at least any one of a date and time of the injection, a term for guarantee of quality, an area where said sample is injected, a depth at which said sample is injected, and guaranteed injection efficiency or expression efficiency.  
     
     
         4 . A method for selling of assigning a plurality of amphibian oocytes comprising, 
 putting a plurality of the amphibian oocytes into which a sample is injected under a substantially same condition in the injection depth into a vessel, and    attaching to said vessel a label on which an information on the injection of the sample into a plurality of said amphibian oocytes is described.    
     
     
         5 . The method for selling or assigning a plurality of the amphibian oocytes according to  claim 4 , wherein said vessel is maintained at a temperature not lower than 18° C. and not higher than 25° C.