Mammary transforming protein

Abstract

A human mammary transforming protein and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for inhibiting such polypeptide for preventing and/or treating neoplasia. Diagnostic assays for identifying mutations in nucleic acid sequence encoding a polypeptide of the present invention and for detecting altered levels of the polypeptide of the present invention for detecting diseases, for example, cancer, are also disclosed.

What is claimed is:  
     
         1 . An isolated polynucleotide comprising a polynucleotide having at least 95% identity to a member selected from the group consisting of: 
 (a) a polynucleotide encoding a polypeptide comprising amino acid 2 to 74 of SEQ ID NO:2; and    (b) the complement of (a).    
     
     
         2 . The isolated polynucleotide of  claim 1  wherein said member is (a).  
     
     
         3 . The isolated polynucleotide of  claim 1  wherein said member is (a) and the polypeptide comprises amino acids 1 to 74 of SEQ ID NO:2.  
     
     
         4 . The isolated polynucleotide of  claim 1  comprising a polynucleotide encoding a polypeptide comprising the amino acid sequence identical to amino acids 2 to 74 of SEQ ID NO:2.  
     
     
         5 . The isolated polynucleotide of  claim 1 , wherein the polynucleotide is DNA.  
     
     
         6 . The isolated polynucleotide of  claim 1  comprising a polynucleotide encoding a polypeptide comprising the amino sequence identical to amino acids 1 to 74 of SEQ ID NO:2.  
     
     
         7 . The isolated polynucleotide of  claim 1 , wherein said polynucleotide is RNA.  
     
     
         8 . A method of making a recombinant vector comprising inserting the isolated polynucleotide of  claim 2  into a vector, wherein said polynucleotide is DNA.  
     
     
         9 . A recombinant vector comprising the polynucleotide of  claim 2 , wherein said polynucleotide is DNA.  
     
     
         10 . A recombinant host cell comprising the polynucleotide of  claim 2 , wherein said polynucleotide is DNA.  
     
     
         11 . A method for producing a polypeptide comprising expressing from the recombinant cell of  claim 10  the polypeptide encoded by said polynucleotide.  
     
     
         12 . A process for producing a polypeptide comprising: 
 expressing from a recombinant cell containing the polynucleotide of  claim 4  the polypeptide encoded by said polynucleotide.    
     
     
         13 . A process for producing a polypeptide comprising: 
 expressing from a recombinant cell containing the polynucleotide of  claim 6  the polypeptide encoded by said polynucleotide.    
     
     
         14 . The isolated polynucleotide of  claim 1  comprising nucleotides 169 to 387 of SEQ ID NO:1.  
     
     
         15 . The isolated polynucleotide of  claim 1  comprising nucleotides 166 to 357 of SEQ ID NO:1.  
     
     
         16 . The isolated polynucleotide of  claim 1  comprising the nucleotides of the sequence of SEQ ID NO:1.  
     
     
         17 . An isolated polynucleotide comprising a polynucleotide having at least a 95% identity to a member selected from the group consisting of: 
 (a) a polynucleotide encoding the same mature polypeptide encoded by the human cDNA in ATCC Deposit No. 97300; and    (b) the complement of (a).    
     
     
         18 . The isolated polynucleotide of  claim 17 , wherein the member is (a).  
     
     
         19 . The isolated polynucleotide of  claim 17 , wherein said polynucleotide comprises DNA identical to the coding portion of the human cDNA in ATCC Deposit No. 97300 which encodes a mature polypeptide.  
     
     
         20 . An isolated polypeptide comprising: 
 a mature polypeptide having an amino acid sequence encoded by a polynucleotide which is at least 95% identical to the polynucleotide of  claim 4 .    
     
     
         21 . The isolated polypeptide of  claim 20 , comprising amino acids 2 to 74 of sequence of SEQ ID NO:2.  
     
     
         22 . The isolated polypeptide of  claim 20 , comprising amino acids 1 to 74 of sequence of SEQ ID NO:2.  
     
     
         23 . The isolated polypeptide of  claim 20  comprising amino acids 1 to 74 of SEQ ID NO:2.  
     
     
         24 . An isolated polypeptide comprising: 
 a mature polypeptide encoded by a polynucleotide which is at least 95% identical to the human cDNA contained in ATCC Deposit No. 97300.    
     
     
         25 . The isolated polypeptide of  claim 24  comprising the mature polypeptide encoded by the human cDNA in ATCC Deposit No. 97300.  
     
     
         26 . An antibody against the polypeptide of  claim 20 .  
     
     
         27 . An antagonist against the polypeptide of  claim 20 .  
     
     
         28 . A method for the treatment of a patient having need of mammary transforming protein comprising: administering to the patient a therapeutically effective amount of the polypeptide of  claim 20 .  
     
     
         29 . The method of  claim 25  wherein said therapeutically effective amount of the polypeptide is administered by providing to the patient DNA encoding said polypeptide and expressing said polypeptide in vivo.  
     
     
         30 . A method for the treatment of a patient having need to inhibit a mammary transforming protein polypeptide comprising: 
 administering to the patient a therapeutically effective amount of the compound of  claim 24 .    
     
     
         31 . A process for diagnosing a disease or a susceptibility to a disease related to an under-expression of the polypeptide of  claim 25  comprising: 
 determining a mutation in a nucleic acid sequence encoding said polypeptide.  
 
     
     
         32 . A diagnostic process comprising: analyzing for the presence of the polypeptide of  claim 25  in a sample derived from a host.  
     
     
         33 . The diagnostic process of  claim 32  wherein said sample is selected from the group consisting of histological sections of mammary sections and blood, and the diagnostic process detects the presence or absence of cancer.  
     
     
         34 . A method for identifying compounds which bind to and inhibit activation of the polypeptide of  claim 25  comprising: 
 contacting a cell expressing on the surface thereof a receptor for the polypeptide, said receptor being associated with a second component capable of providing a detectable signal in response to the binding of a compound to said receptor, with an analytically detectable mammary transforming protein and a compound under conditions to permit binding to the receptor; and  
 determining whether the compound binds to and inhibits the receptor by detecting the absence of a signal generated from the interaction of the mammary transforming protein with the receptor.