Method of producing L-serine by fermentation

Abstract

Disclosed is a coryneform bacterium having resistance to azaserine or β-(2-thienyl)-DL-alanine and having L-serine productivity. Also, disclosed are D-3-phosphoglycerate dehydrogenase derived from a coryneform bacterium, in which feedback inhibition by L-serine is desensitized; the D-3-phosphoglycerate dehydrogenase, which is obtainable from a coryneform bacterium having resistance to azaserine or β-(2-thienyl)-DL-alanine and having L-serine productivity; the D-3-phosphoglycerate dehydrogenase having an amino acid sequence depicted in SEQ ID NO: 12 in Sequence Listing or the sequence including substitution, addition or deletion of one or more amino acids, wherein an amino acid residue corresponding to the 325th glutamic acid residue of the amino acid sequence in the SEQ ID NO: 12 is replaced with an amino acid other than glutamic acid; the D-3-phosphoglycerate dehydrogenase that has an amino acid sequence depicted in SEQ ID NO: 11 in Sequence Listing; a DNA coding for the D-3-phosphoglycerate dehydrogenase described above; the DNA that has a base sequence depicted in SEQ ID NO: 13 in Sequence Listing; a coryneform bacterium which harbors a recombinant DNA containing the DNA; and a method of producing L-serine, comprising the steps of cultivating the bacterium described above in a medium to allow accumulation of L-serine in the medium, and collecting the L-serine from the medium.

What is claimed is:  
     
         1 . A coryneform bacterium having resistance to azaserine or β-(2-thienyl)-DL-alanine and having L-serine productivity.  
     
     
         2 . D-3-phosphoglycerate dehydrogenase derived from a coryneform bacterium, in which feedback inhibition by L-serine is desensitized.  
     
     
         3 . The D-3-phosphoglycerate dehydrogenase as claimed in  claim 2 , which is obtainable from a coryneform bacterium having resistance to azaserine or β-(2-thienyl)-DL-alanine and having L-serine productivity.  
     
     
         4 . The D-3-phosphoglycerate dehydrogenase as claimed in  claim 2 , having an amino acid sequence depicted in SEQ ID NO: 12 in Sequence Listing or said sequence including substitution, addition or deletion of one or more amino acids, wherein an amino acid residue corresponding to the 325th glutamic acid residue of the amino acid sequence in the SEQ ID NO: 12 is replaced with an amino acid other than glutamic acid.  
     
     
         5 . The D-3-phosphoglycerate dehydrogenase as claimed in  claim 2 , which has an amino acid sequence depicted in SEQ ID NO: 11 in Sequence Listing.  
     
     
         6 . A DNA coding for the D-3-phosphoglycerate dehydrogenase as claimed in any one of  claims 2  to  5 .  
     
     
         7 . The DNA as claimed in  claim 6 , wherein said DNA has a base sequence depicted in SEQ ID NO: 13 in Sequence Listing.  
     
     
         8 . A coryneform bacterium which harbors a recombinant DNA containing the DNA as claimed in  claim 6 .  
     
     
         9 . A method of producing L-serine, comprising the steps of cultivating the bacterium as claimed in  claim 1  or  8  in a medium to allow accumulation of L-serine in the medium, and collecting the L-serine from the medium.