Cellulase for use in industrial processes

Abstract

A method for treating cellulosic materials is disclosed which comprises contacting the cellulosic material with a cellulase obtainable from Thermomonospora fusca corresponding to E5 or a derivative thereof. Particularly preferred methods comprise stonewashing and detergent cleaning of cotton fabrics, the production of paper products, as an additive to animal feed and in the production of food, starch, ethanol and sugar.

We claim:  
     
         1 . A method of treating cellulosic material comprising contacting said material with a cellulase obtainable from  Thermomonospora fusca  corresponding to E5, a truncated E5, or a derivative thereof.  
     
     
         2 . The method according to  claim 1 , wherein said truncated E5 is obtained through proteolytic cleavage.  
     
     
         3 . The method according to  claim 1 , wherein said truncated E5 is obtained through genetic engineering techniques.  
     
     
         4 . The method according to  claim 1 , wherein said cellulosic material comprises cellulose containing fabric.  
     
     
         5 . The method according to  claim 4 , wherein said cellulose containing fabric is treated so as to effect a stonewashed effect.  
     
     
         6 . The method according to  claim 4 , wherein said cellulose containing fabric is treated so as to improve the appearance and/or feel of said fabric.  
     
     
         7 . The method according to  claim 4 , wherein said cellulase is incorporated into a detergent composition.  
     
     
         8 . The method according to  claim 6 , wherein said cellulose containing fabric comprises a faded, colored fabric and said improvement comprises improving the appearance of said faded colored fabric by rejuvenating the color thereof.  
     
     
         9 . The method according to  claim 6 , wherein said improvement comprises a softer or smoother feel to said fabric.  
     
     
         10 . The method according to  claim 1 , wherein said cellulosic material comprises wood pulp.  
     
     
         11 . The method according to  claim 1 , wherein said cellulosic material comprises animal feed or grain.  
     
     
         12 . A stonewashing composition comprising a cellulase selected from the group consisting of E5, truncated E5, or a derivative thereof.  
     
     
         13 . The composition according to  claim 12 , further comprising a surfactant.  
     
     
         14 . The composition according to  claim 12 , wherein said composition is a concentrate.  
     
     
         15 . A detergent composition comprising a cellulase selected from the group consisting of E5, truncated E5, or a derivative thereof.  
     
     
         16 . The detergent composition according to  claim 15 , wherein said cellulase is present in a concentration of at least 10 ppm.  
     
     
         17 . A feed additive comprising E5, truncated E5, or a derivative thereof.  
     
     
         18 . The composition according to claims  12  or  15 , wherein said truncated E5 differs from E5 by having any segment of amino acid residues 1-120 of said E5 deleted.  
     
     
         19 . The detergent composition according to  claim 15 , wherein said detergent is a liquid.