US2002187489A1PendingUtilityA1

Method for screening for enzyme activity

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Assignee: DIVERSA CORPPriority: Dec 7, 1995Filed: Feb 5, 2002Published: Dec 12, 2002
Est. expiryDec 7, 2015(expired)· nominal 20-yr term from priority
Inventors:Jay M. Short
C12N 9/16C12N 15/1034C12N 9/00C12N 15/102C12Q 1/6813C12N 9/1029
54
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Claims

Abstract

Disclosed is a process for identifying clones having a specified enzyme activity by screening for the specified enzyme activity in a library of clones prepared by (i) selectively isolating target DNA from DNA derived from at least one microorganism, by use of at least one probe DNA comprising at least a portion of a DNA sequence encoding an enzyme having the specified enzyme activity; and (ii) transforming a host with isolated target DNA to produce a library of clones which are screened for the specified enzyme activity.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A method for enriching for DNA sequences containing at least a partial coding region for at least one specified protein activity in a DNA sample comprising selecting and recovering a mixture of target DNA from a mixture of organisms, by use of a mixture of DNA probes comprising at least a portion of a DNA sequence encoding at least one protein having a specified protein activity.

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