US2002176848A1PendingUtilityA1

Antimicrobial mediated bacterial DNA delivery

49
Priority: Mar 28, 1997Filed: May 16, 2002Published: Nov 28, 2002
Est. expiryMar 28, 2017(expired)· nominal 20-yr term from priority
Inventors:Donata R. SizemoreJerald C. SadoffJason C. Grove
A61K 48/00C12N 15/74C12N 15/87A61K 48/0008
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PatentIndex Score
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Claims

Abstract

This invention pertains in part to a method for delivering functional DNA or antigens. The desired DNA is introduced into attenuated bacteria able to enter cells. Once the bacteria is in the cell, antimicrobial agents are introduced such that they enter the mammalian cell and lyse the bacteria thereby allowing the delivery of carried functional DNA or antigens. The advantages of this method and its uses are described.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A method for delivering functional DNA into a cell, said method comprising the steps of: 
 (i) introducing said functional DNA into an attenuated bacteria able to enter cells;    (ii) allowing said attenuated bacteria to enter cell; and    (iii) lysing said bacteria inside said cell such that said DNA is released into said cell.    
     
     
         2 . A method for the delivery of functional DNA to a cell according to  claim 1 , wherein said bacteria is Shigella.  
     
     
         3 . A method for the delivery of functional DNA to a cell according to  claim 2  wherein said Shigella is SC602.  
     
     
         4 . A method for the delivery of functional DNA to a cell according to  claim 1 , wherein lysing of said bacteria in step (iii) is by using an antimicrobial.  
     
     
         5 . A method for the delivery of functional DNA to cell according to  claim 4 , wherein said antimicrobial is an antibiotic.  
     
     
         6 . A method for the delivery of functional DNA to a cell according to  claim 5 , wherein said antibiotic is azithromycin.  
     
     
         7 . A method for the delivery of functional DNA to a cell according to  claim 1 , wherein said cell is a cell of an intestinal mucosal epithelium cell.  
     
     
         8 . A method for delivering functional DNA to a cell according to  claim 7 , wherein said mucosal epithelium is intestinal mucosal epithelium.  
     
     
         9 . A method for delivering an antigen to a cell comprising: 
 (i) introducing said antigen into an attenuated bacteria able to enter cells;    (ii) allowing said attenuated bacteria to enter cell; and    (iii) lysing said bacteria inside said cell such that said antigen is released into said cell.    
     
     
         10 . A DNA delivery kit for the delivery of functional DNA into cells, said kit comprising attenuated Shigella containing said DNA, and an antimicrobial able to enter said cells and lyse said attenuated Shigella.

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