Membrane associated protein zupar1

Abstract

Novel membrane associated polypeptides, polynucleotides encoding those polypeptides, related compositions, antibodies and methods are disclosed.

I claim:  
     
         1 . An isolated polypeptide, comprising an amino acid sequence of at least 15 contiguous amino acid residues of SEQ ID NO:2.  
     
     
         2 . The isolated polypeptide of  claim 1 , wherein the isolated polypeptide comprises at least 20 contiguous amino acid residues of SEQ ID NO:2.  
     
     
         3 . The isolated polypeptide of  claim 1 , wherein the isolated polypeptide comprises at least 30 contiguous amino acid residues of SEQ ID NO:2.  
     
     
         4 . The isolated polypeptide of  claim 1 , wherein the isolated polypeptide is selected from the group consisting of: 
 a) the polypeptide of SEQ ID NO:6;    b) the polypeptide of SEQ ID NO:7; or    c) the polypetide of SEQ ID NO:8.    
     
     
         5 . The isolated polypeptide of  claim 1 , wherein the isolated polypeptide is selected from the group consisting of: 
 a) the polypeptide of SEQ ID NO:9;    b) the polypeptide of SEQ ID NO:10; or    c) the polypetide of SEQ ID NO:11.    
     
     
         6 . The isolated polypeptide of  claim 1 , comprising the amino acid sequence of SEQ ID NO:2.  
     
     
         7 . An isolated nucleic acid molecule, wherein the nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:3, (b) a nucleic acid molecule encoding the amino acid sequence of SEQ ID NO:2, and (c) a nucleic acid molecule that remains hybridized following stringent wash conditions to a nucleic acid molecule consisting of a nucleotide sequence of SEQ ID NO:1, or the complement of a nucleotide sequence of SEQ ID NO:1.  
     
     
         8 . The isolated nucleic acid molecule of  claim 7 , wherein any difference between the amino acid sequence encoded by the nucleic acid molecule and the corresponding amino acid sequence of SEQ ID NO:2 is due to a conservative amino acid substitution.  
     
     
         9 . The isolated nucleic acid molecule of  claim 7 , comprising the nucleotide sequence of SEQ ID NO:1.  
     
     
         10 . A vector, comprising the isolated nucleic acid molecule of  claim 9 .  
     
     
         11 . An expression vector, comprising the isolated nucleic acid molecule of  claim 9 , a transcription promoter, and a transcription terminator, wherein the promoter is operably linked with the nucleic acid molecule, and wherein the nucleic acid molecule is operably linked with the transcription terminator.  
     
     
         12 . A recombinant host cell comprising the expression vector of  claim 11 , wherein the host cell is selected from the group consisting of bacterium, yeast cell, fungal cell, insect cell, avian cell, mammalian cell, and plant cell.  
     
     
         13 . A method of using the expression vector of  claim 11  to produce zupar1 protein, comprising culturing recombinant host cells that comprise the expression vector and that produce the zupar1 protein.  
     
     
         14 . The method of  claim 13 , further comprising isolating the zupar1 protein from the cultured recombinant host cells.  
     
     
         15 . An antibody or antibody fragment that specifically binds with the polypeptide of  claim 6 .  
     
     
         16 . The antibody of  claim 15 , wherein the antibody is selected from the group consisting of: (a) polyclonal antibody, (b) murine monoclonal antibody, (c) humanized antibody derived from (b), and (d) human monoclonal antibody.  
     
     
         17 . An anti-idiotype antibody that specifically the binds the antibody of  claim 15 .  
     
     
         18 . A method of detecting the presence of zupar1 gene expression in a biological sample, comprising: 
 (a) contacting a zupar1 nucleic acid probe under hybridizing conditions with either (i) test RNA molecules isolated from the biological sample, or (ii) nucleic acid molecules synthesized from the isolated RNA molecules, wherein the probe consists of a nucleotide sequence comprising a portion of the nucleotide sequence of the nucleic acid molecule of  claim 8 , or complements thereof, and    (b) detecting the formation of hybrids of the nucleic acid probe and either the test RNA molecules or the synthesized nucleic acid molecules, wherein the presence of the hybrids indicates the presence of zupar1 RNA in the biological sample, or,    (a′) contacting the biological sample with an antibody, or an antibody fragment, which specifically binds with a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, wherein the contacting is performed under conditions that allow the binding of the antibody or antibody fragment to the biological sample, and    (b′) detecting any of the bound antibody or bound antibody fragment.    
     
     
         19 . A composition, comprising a carrier and a polypeptide selected from the group consisting of: 
 a) the polypeptide of SEQ ID NO:6;    b) the polypeptide of SEQ ID NO:7;    c) the polypetide of SEQ ID NO:8;    d) the polypeptide of SEQ ID NO:9;    e) the polypeptide of SEQ ID NO:10; or    f) the polypetide of SEQ ID NO:11.    
     
     
         20 . A fusion protein, comprising a polypeptide selected from the group consisting of: 
 a) the polypeptide of SEQ ID NO:6;    b) the polypeptide of SEQ ID NO:7;    c) the polypeptide of SEQ ID NO:8;    d) the polypeptide of SEQ ID NO:9;    e) the polypeptide of SEQ ID NO:10; or    f) the polypetide of SEQ ID NO:11.