Adsorbents for high mobility group proteins and column for purifying body fluid

Abstract

An adsorbent of high-mobility-group proteins (HMG protein) which can remove HMG protein in body fluid is disclosed. The adsorbent according to the present invention comprises a water-insoluble carrier on which (a) substance(s) having (a) hydrogen-bondable functional group(s) and/or (a) hydrophobic functional group(s) is(are) immobilized.

1 . An adsorbent of high-mobility-group proteins comprising a water-insoluble carrier on which (a) substance(s) having (a) hydrogen-bondable functional group(s) and/or (a) hydrophobic functional group(s) is(are) immobilized.  
     
     
         2 . The adsorbent according to  claim 1 , wherein said hydrogen-bondable functional group(s) is(are) (a) cationic functional group(s).  
     
     
         3 . The adsorbent according to  claim 2 , wherein said cationic functional group(s) is(are) primary amino group, secondary amino group, tertiary amino group, imino group and/or quaternary ammonium group.  
     
     
         4 . The adsorbent according to  claim 3 , wherein said cationic functional group(s) is(are) represented by the following Formula (I):  
       
         
           
           
               
               
           
         
       
       (wherein R 1 , R 2  and R 3  independently represent hydrogen atom or a C 1 -C 5  alkyl group, respectively)  
     
     
         5 . The adsorbent according to  claim 4 , wherein in Formula (I), R 1 , R 2  and R 3  independently represent hydrogen atom or a C 1 -C 2  alkyl group, respectively.  
     
     
         6 . The adsorbent according to  claim 5 , wherein all of R 1 , R 2  and R 3  in Formula (I) represent methyl group.  
     
     
         7 . The adsorbent according to  claim 1 , wherein said hydrogen-bondable functional group(s) is(are) (an) anionic functional group(s).  
     
     
         8 . The adsorbent according to  claim 7 , wherein said hydrogen-bondable functional group(s) is(are) carboxyl group, sulfate group, sulfonic acid group, and/or phosphate group.  
     
     
         9 . The adsorbent according to  claim 1 , wherein said hydrophobic functional group(s) is(are) (an) alkyl group(s) having not less than 6 carbon atoms, or (an) aromatic group(s).  
     
     
         10 . The adsorbent according to  claim 1 , wherein said substance(s) having (a) hydrogen-bondable functional group(s) and/or (a) hydrophobic functional group(s) is(are) (a) peptide(s) or (an) amino acid(s).  
     
     
         11 . The adsorbent according to  claim 3 , wherein said substance(s) having (a) hydrogen-bondable functional group(s) is(are) (a) peptide(s) or (an) amino acid(s) having (an) amino group(s) in its(their) side chain(s).  
     
     
         12 . The adsorbent according to  claim 11 , wherein said substance having (a) hydrogen-bondable functional group(s) is(are) polylysine.  
     
     
         13 . The adsorbent according to  claim 7 , wherein (a) polysaccharide(s) having (an) sulfate group(s) is(are) immobilized.  
     
     
         14 . The adsorbent according to  claim 13 , wherein said polysaccharide is heparin or dextran sulfate, or a derivative thereof.  
     
     
         15 . An adsorbent of high-mobility-group proteins comprising a water-insoluble carrier on which (an) antibody(ies) to said high-mobility-group proteins is(are) immobilized.  
     
     
         16 . The adsorbent according to  claim 15 , wherein said adsorbent has a rate of adsorption for high-mobility-group proteins of not less than 50%, and has a rate of adsorption for serum albumin of not more than 20%.  
     
     
         17 . The adsorbent according to any one of  claims 1  to  16 , wherein said water-insoluble carrier is in the form of fibers.  
     
     
         18 . The adsorbent according to any one of  claims 1  to  16 , wherein said water-insoluble carrier is in the form of beads.  
     
     
         19 . The adsorbent according to any one of  claims 1  to  18 , which is used for therapy of sepsis.  
     
     
         20 . A body fluid-purification column for removing high-mobility-group proteins, comprising a column, and said adsorbent according to any one of  claims 1  to  19 , which adsorbent is packed in said column.  
     
     
         21 . The body fluid-purification column according to  claim 20 , by which extracorporeal circulation of whole blood can be attained.  
     
     
         22 . The body fluid-purification column according to  claim 20  or  21 , which is used for therapy of sepsis.  
     
     
         23 . A method for adsorbing high-mobility-group proteins in body fluid, comprising contacting said adsorbent according to any one of  claims 1  to  18  with body fluid so as to adsorb said high-mobility-group proteins in said body fluid to said adsorbent.  
     
     
         24 . The method according to  claim 23 , which is carried out by using said body fluid-purification column according to any one of  claims 20  to  22 .  
     
     
         25 . The method according to  claim 23  or  24 , wherein said body fluid is blood.  
     
     
         26 . The method according to any one of  claims 24  to  25 , which is carried out for therapy of sepsis.  
     
     
         27 . The method according to  claim 26 , which is carried by using said body fluid-purification column according to any one of  claims 20  to  22  together with (a) body fluid-purification column(s) which adsorb(s) (a) substance(s) originated from bacteria.  
     
     
         28 . Use of said adsorbent according to any one of  claims 1  to  18  for production of adsorbent material for adsorbing high-mobility-group proteins in body fluid.  
     
     
         29 . The use according to  claim 27 , wherein said body fluid is blood.  
     
     
         30 . The use according to  claim 27  or  28 , wherein said adsorbent material is for therapy of sepsis.