Cannine androgen receptor

Abstract

The present invention provides isolated canine AR protein, DNA molecules encoding said protein, vectors and cells carrying said DNA molecules, specific bind partners for the protein, and methods of discovering ligands for canine AR.

That which is claimed is:  
     
         1 . An isolated proteinaceous molecule having canine AR activity, wherein said proteinaceous molecule comprises an amino acid sequence of SEQ ID NO:2, or an amino acid sequence of SEQ ID NO:2 with one or more conservative substitutions therein.  
     
     
         2 . An isolated proteinaceous molecule according to  claim 1 , wherein said amino acid sequence has zero to three conservative substitutions therein.  
     
     
         3 . An isolated proteinaceous molecule according to  claim 1 , wherein said amino acid sequence comprises an amino acid sequence of SEQ ID NO:2.  
     
     
         4 . An isolated DNA molecule encoding a proteinaceous molecule according to  claim 1 .  
     
     
         5 . An isolated DNA molecule encoding a proteinaceous molecule according to  claim 3 .  
     
     
         6 . A recombinant expression vector comprising a DNA molecule according to  claim 4 .  
     
     
         7 . A cell transformed by an expression vector according to  claim 6 .  
     
     
         8 . A cell according to  claim 7 , wherein said cell is that of a prokaryote.  
     
     
         9 . A cell according to  claim 7 , wherein said cell is that of a eukaryote.  
     
     
         10 . A cell according to  claim 9 , wherein said eukaryote is yeast.  
     
     
         11 . A cell according to  claim 9 , wherein said eukaryote is a mammal.  
     
     
         12 . A cell according to  claim 11 , which is a CHO cell.  
     
     
         13 . A method of producing a proteinaceous molecule having an activity of canine AR, which comprises culturing a cell according to  claim 7  for a time sufficient to produce said proteinaceous molecule.  
     
     
         14 . A pharmaceutical composition comprising the proteinaceous molecule according to  claim 1  and a pharmaceutically acceptable carrier therefor.  
     
     
         15 . A pharmaceutical composition comprising the proteinaceous molecule according to  claim 3  and a pharmaceutically acceptable carrier therefor.  
     
     
         16 . A specific binding partner that selectively binds to the proteinaceous molecule according to  claim 1 .  
     
     
         17 . A method of discovering ligands for the canine AR protein, said method comprising the steps of: 
 a) combining a test substance with the proteinaceous molecule according to  claim 1;     b) measuring specific binding between said test substance and said proteinaceous molecule; and    c) classifying as a ligand said test substance if it binds to said proteinaceous molecule.    
     
     
         18 . A method of discovering modulators of canine AR protein activity, said method comprising the steps of: 
 a) combining a test substance with the proteinaceous molecule according to  claim 1;     b) measuring the activity of said proteinaceous molecule in the presence and absence of said test substance and; and    c) classifying said test substance as a modulator of canine AR activity if it modulates the activity of said proteinaceous molecule.