Novel semaphorin genes (I)

Abstract

The present invention provides a novel Semaphorin having neurite-outgrowth inhibition activity or proteins analogous thereto, peptide fragments of, or antibodies against, such proteins, genes encoding such proteins, expression vectors for said genes, transformed cells into which said expression vectors have been introduced, methods for producing a recombinant protein which employ said transformed cells, antisense nucleotides against the above genes, transgenic animals involving insertion or deletion of the above genes, and screening methods for antagonists of the above proteins, all of which are useful mainly in diagnoses, treatments, or studies relating to neurological diseases. The present invention further provides use of such proteins, peptides, antibodies, genes, or antisense nucleotides as pharmaceutical or diagnostic agents or laboratory reagents.

1 . An isolated polypeptide comprising the amino acid sequence show in SEQ ID NO:1.  
     
     
         2 . An isolated polypeptide comprising six or more contiguous amino acids of SEQ ID NO:1.  
     
     
         3 . A polypeptide obtained by expressing in a cell a nucleic acid comprising a polynucleotide having a nucleotide sequence selected from the group consisting of: 
 (a) the nucleotide sequence of SEQ ID NO:2;    (b) the nucleotide sequence of SEQ ID NO:2 from nucleotide 370 to nucleotide 2694; and    (c) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1.    
     
     
         4 . A polypeptide obtained by expressing in a cell a nucleic acid comprising a polynucleotide that specifically hybridizes with the complement of a polynucleotide having a nucleotide sequence selected from the group consisting of: 
 (a) the nucleotide sequence of SEQ ID NO:2; and    (b) the nucleotide sequence of SEQ ID NO:2 from nucleotide 370 to nucleotide 2694;    under hybridization conditions of a buffer comprising 45%(v/v) formamide, 5×SSPE at 42° C., and washing after hybridization with a buffer comprising 0.1×SSC, 0.1% SDS at 65° C.,    wherein said polypeptide has the biological activity of inhibiting neurite outgrowth from dorsal root ganglion cells.    
     
     
         5 . A polypeptide obtained by expressing in a cell a nucleic acid comprising a polynucleotide that specifically hybridizes with the complement of a polynucleotide having a nucleotide sequence selected from the group consisting of: 
 (a) the nucleotide sequence of SEQ ID NO:2; and    (b) the nucleotide sequence of SEQ ID NO:2 from nucleotide 370 to nucleotide 2694;    under hybridization conditions of a buffer comprising 45%(v/v) formamide, 5× SSPE at 42° C., and washing after hybridization with a buffer comprising 0.1× SSC, 0.1% SDS at 65° C., wherein said polypeptide has the biological activity of collapsing growth cones of neuron cells.