US2002142467A1PendingUtilityA1

Method for culturing recombinant cells

60
Assignee: GENENTECH INCPriority: Sep 11, 1987Filed: Apr 22, 2002Published: Oct 3, 2002
Est. expirySep 11, 2007(expired)· nominal 20-yr term from priority
Inventors:Jennie P. MatherAxel Ullrich
C07K 14/79C07K 14/59
60
PatentIndex Score
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Cited by
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References
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Claims

Abstract

A method for culturing a recombinant host cell comprising: determining a polypeptide factor for a polypeptide factor-dependent host cell; transforming said host cell with nucleic acid encoding said polypeptide factor; transforming the host cell with nucleic acid encoding a desired protein; and, culturing the transformed host cells in a medium lacking the polypeptide factor.

Claims

exact text as granted — not AI-modified
1 . A method for culturing a host cell comprising: 
 a. determining a polypeptide factor for a polypeptide factor-dependent host cell;    b. transforming said host cell with nucleic acid encoding said polypeptide factor:    c. transforming the host cell with nucleic acid encoding a desired protein; and    d. culturing the transformed cells of step (c) in a medium lacking the polypeptide factor.    
     
     
         2 . The method of  claim 1  which additionally comprises the step of recovering the desired protein.  
     
     
         3 . The method of  claim 1  wherein the medium is serum-free medium.  
     
     
         4 . The method of  claim 1  wherein the host cell is a vertebrate host cell.  
     
     
         5 . The method of  claim 1  wherein the host cell is a chinese hamster ovary cell.  
     
     
         6 . The method of  claim 1  wherein the polypeptide factor is proinsulin.  
     
     
         7 . The method of  claim 1  wherein the polypeptide factor is transferrin.  
     
     
         8 . The method of  claim 1  wherein the polypeptide factor is transferrin and insulin.  
     
     
         9 . A host cell transformed to express a desired protein and a polypeptide factor.  
     
     
         10 . The host cell of  claim 9  which is a vertebrate cell.  
     
     
         11 . The host cell of  claim 9  which is a chinese hamster ovary cell.  
     
     
         12 . The host cell of  claim 11  wherein the polypeptide factor is proinsulin.  
     
     
         13 . The host cell of  claim 9  which is a 293 cell.  
     
     
         14 . The host cell of  claim 9  wherein the polypeptide factor is proinsulin.  
     
     
         15 . The host cell of  claim 9  wherein the polypeptide factor is transferrin.  
     
     
         16 . A culture comprising the host cell of  claim 9  and a medium lacking the polypeptide factor expressed by said host cell.  
     
     
         17 . The culture of  claim 16  wherein the medium is a serum-free medium.  
     
     
         18 . The culture of  claim 16  wherein the polypeptide factor lacking in the medium is insulin.

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