US2002137200A1PendingUtilityA1

Biosensor and blood component analyzing method

46
Priority: Jun 1, 2000Filed: Jun 1, 2001Published: Sep 26, 2002
Est. expiryJun 1, 2020(expired)· nominal 20-yr term from priority
Inventors:Mie TakahashiMasataka NadaokaHirotaka TanakaFumihisa Kitawaki
G01N 33/5005Y10S436/81G01N 30/90Y10S436/808G01N 33/5002G01N 33/54388G01N 33/54391G01N 33/54387
46
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Claims

Abstract

According to the biosensor and the blood component analytical method of the present invention, in a biosensor that is made of a single layer or plural layers of a porous material as shown in FIG. 1 , having a reagent holding part and utilizing chromatography, a cell shrinkage reagent is carried on at least part of the reagent holding part, or at least part of a chromatographically developed part that is upstream of the reagent holding part. According to the biosensor having the above-mentioned structure and the blood component analytical method, even when whole blood is a sample, a high-accuracy blood component analysis can be performed easily and quickly with less cost.

Claims

exact text as granted — not AI-modified
1 . A biosensor that is made of a single layer or plural layers of a porous material, said biosensor having a reagent holding part and utilizing chromatography, wherein 
 a cell shrinkage reagent is carried on at least part of the reagent holding part, or at least part of a chromatographically developed part which is upstream of the reagent holding part.    
     
     
         2 . The biosensor of  claim 1  wherein 
 a liquid specimen to be added is whole blood.  
 
     
     
         3 . The biosensor of  claim 1  wherein 
 a liquid specimen to be added is a solution including bacteria.  
 
     
     
         4 . The biosensor of  claim 1  wherein 
 the cell shrinkage reagent is inorganic salt.  
 
     
     
         5 . The biosensor of  claim 1  wherein 
 the cell shrinkage reagent is amino acid.  
 
     
     
         6 . The biosensor of  claim 1  wherein 
 the cell shrinkage reagent is saccharide.  
 
     
     
         7 . The biosensor of  claim 1  wherein 
 a carrier that carries the cell shrinkage reagent is dried naturally or dried by air-drying.  
 
     
     
         8 . The biosensor of  claim 1  wherein 
 a carrier that carries the cell shrinkage reagent is dried by freeze-drying.  
 
     
     
         9 . The biosensor of  claim 1  wherein 
 a carrier that carries the cell shrinkage reagent is dried by heat drying.  
 
     
     
         10 . The biosensor of  claim 1  wherein 
 the biosensor is a one-step immunochromatographic test strip.  
 
     
     
         11 . The biosensor of  claim 1  wherein 
 the biosensor is a dry analytical element.  
 
     
     
         12 . A blood component analytical method in which a biosensor that is made of a single layer or plural layers of a porous material, said biosensor having a reagent holding part and utilizing chromatography is employed, wherein 
 cell components shrink and the shrunk cell components are separated in an area of at least part of the reagent holding part, or at least part of a chromatographically developed part that is upstream of the reagent holding part, on which a cell shrinkage reagent is carried.    
     
     
         13 . The blood component analytical method of  claim 12  wherein 
 a blood specimen to be added is whole blood.  
 
     
     
         14 . The blood component analytical method of  claim 12  wherein 
 the cell shrinkage reagent is inorganic salt.  
 
     
     
         15 . The blood component analytical method of  claim 12  wherein 
 the cell shrinkage reagent is amino acid.  
 
     
     
         16 . The blood component analytical method of  claim 12  wherein 
 the cell shrinkage reagent is saccharide.  
 
     
     
         17 . The blood component analytical method of  claim 12  wherein 
 a carrier that carries the cell shrinkage reagent is dried naturally or dried by air-drying.  
 
     
     
         18 . The blood component analytical method of  claim 12  wherein 
 a carrier that carries the cell shrinkage reagent is dried by freeze-drying.  
 
     
     
         19 . The blood component analytical method of  claim 12  wherein 
 a carrier that carries the cell shrinkage reagent is dried by heat drying.  
 
     
     
         20 . The blood component analytical method of  claim 12  wherein 
 the concentration of the cell shrinkage reagent is 0.05˜0.3M.  
 
     
     
         21 . The blood component analytical method of  claim 12  wherein 
 the biosensor is a one-step immunochromatographic test strip.  
 
     
     
         22 . The blood component analytical method of  claim 12  wherein 
 the biosensor is a dry analytical element.  
 
     
     
         23 . A blood component analytical method in which a biosensor 
 that is made of a single layer or plural layers of a porous material, said biosensor having a reagent holding part and utilizing chromatography is employed, wherein    cell components shrink or shrink while being chromatographically developed in a state where shrunk cell components are mixed, in an area of at least part of the reagent holding part, or at least part of a chromatographically developed part that is upstream of the reagent holding part, on which a cell shrinkage reagent is carried.    
     
     
         24 . The blood component analytical method of  claim 23  wherein 
 a blood specimen to be added is whole blood.  
 
     
     
         25 . The blood component analytical method of  claim 23  wherein 
 the cell shrinkage reagent is inorganic salt.  
 
     
     
         26 . The blood component analytical method of  claim 23  wherein 
 the cell shrinkage reagent is amino acid.  
 
     
     
         27 . The blood component analytical method of  claim 23  wherein 
 the cell shrinkage reagent is saccharide.  
 
     
     
         28 . The blood component analytical method of  claim 23  wherein 
 a carrier that carries the cell shrinkage reagent is dried naturally or dried by air-drying.  
 
     
     
         29 . The blood component analytical method of  claim 23  wherein 
 a carrier that carries the cell shrinkage reagent is dried by freeze-drying.  
 
     
     
         30 . The blood component analytical method of  claim 23  wherein 
 a carrier that carries the cell shrinkage reagent is dried by heat-drying.  
 
     
     
         31 . The blood component analytical method of  claim 23  wherein 
 the concentration of the cell shrinkage reagent is 0.1˜5.0m.  
 
     
     
         32 . The blood component analytical method of  claim 23  wherein 
 the biosensor is a one-step immunochromatographic test strip.  
 
     
     
         33 . The blood component analytical method of  claim 23  wherein 
 the biosensor is a dry analytical element.

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