US2002137155A1PendingUtilityA1
Process for producing, methods and compositions of glucuronoxylomannan as nutriceutical agent from higher Basidiomycetes mushroom
Priority: Oct 15, 1999Filed: Feb 26, 2002Published: Sep 26, 2002
Est. expiryOct 15, 2019(expired)· nominal 20-yr term from priority
A61P 9/10A61P 43/00A61P 31/00A61P 35/00C12N 1/14C12P 1/02A61P 25/32A61K 36/07
41
PatentIndex Score
0
Cited by
0
References
0
Claims
Abstract
The present invention describes new and distinct strains of higher Basidiomycetes mushrooms, and a process for growing them in submerged culture. Specifically, the new strains of species of the genus Pleurotus offer superior yields of mushroom biomass and concentrations of biologically active compounds, for example, cholesterol-lowering compounds, lectins, proteins, essential amino acids, vitamins or polysaccharides. The process includes use of defined media and a simple one-step procedure of separating the lovastatin-containing nutriceuticals from culture broth.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . A process for producing a biomass of edible Basidiomycetes mushrooms, said process comprising: cultivating a fungi of the Basidiomycetes in a submerged culture on nutrient media, isolating the resulting biomass of edible Basidiomycetes from the culture broth, and drying the biomass of edible Basidiomycetes at a temperature range of 40° C. to 45° C.
2 . The process for producing a biomass of edible Basidiomycetes mushrooms according to claim 1 , wherein the fungi of the Basidiomycetes is a species of the genus Pleurotus comprising Pleurotus eryngii var. ferulae or Pleurotus ostreatus.
3 . The process for producing a biomass of edible Basidiomycetes mushrooms according to claim 1 , wherein the fungi of the Basidiomycetes produces lovastatin.
4 . The process for producing a biomass of edible Basidiomycetes mushrooms according to claim 1 , wherein the nutrient media is of the following composition (g/l):
Medium A
Glucose (Dextrose)-
25
Peptone-
2
Yeast extract-
1
KH 2 PO4—
0.5
MgSO 4 .7H 2 O—
0.25
CaCl 2 .2H 2 O—
0.1
Corn Steep Liquor-
2.5
ml
Trace Element Mixture
10
ml
10% KOH -
2.5
ml
Trace Element Mixture:
FeSO 4 .7H2O—
0.5
MnSO 4 .H 2 O—
0.1
ZnSO 4 .7H 2 O—
0.05
CuSO 4 .5H 2 O—
0.02
5 . The process for producing a biomass of edible Basidiomycetes mushrooms according to claim 1 , wherein the nutrient media is of the following composition (g/l):
Medium B
Glucose (Dextrose)-
50
Peptone-
0.5
Yeast extract-
1
(NH 4 ) 2 SO 4 —
2
K 2 HPO 4 .3H 2 O—
2
K 2 HPO 4
2.5
Trace Element Mixture -
10
ml
Trace Element Mixture (g/l):
FeSO 4 .7H 2 O—
0.5
MnSO 4 .H 2 O—
0.1
ZnSO 4 .7H 2 O—
0.05
CuSO 4 .5H 2 O—
0.02
6 . The process for producing a biomass of edible Basidiomycetes mushrooms according to claim 1 , wherein the fungus is Pleurotus eryngii var. ferulae CBS 101938.
7 . The process for producing a biomass of edible Basidiomycetes mushrooms according to claim 1 , wherein the fungus is Pleurotus ostreatus CBS 101937.
8 . A composition for reducing blood levels of cholesterol in a mammal, said composition comprising a nutriceutical formulation of dried Basidiomycetes biomass.
9 . The composition according to claim 8 wherein the dried Basidiomycetes biomass comprises a cholesterol-lowering compound, a lectin, polysaccharide, fiber, protein, essential amino acids, vitamins, fatty acids, or minerals.
10 . The composition according to claim 8 , wherein the dried Basidiomycetes biomass is produced from Pleurotus eryngii var. ferulae CB 101938.
11 . The composition according to claim 8 , wherein the dried Basidiomycetes biomass is produced from Pleurotus ostreatus CB 101937.
12 . The composition according to claim 8 wherein the dried Basidiomycetes biomass is in an effective amount to reduce blood levels of cholesterol, in combination with a pharmaceutical carrier acceptable for oral administration.
13 . The composition according to claim 8 wherein the dried Basidiomycetes biomass is in an effective amount to inhibit atherosclerosis.
14 . The composition according to claim 8 wherein the dried Basidiomycetes biomass is in an effective amount to inhibit a variety of diseases including cardiovascular disease, cancer, hepatotoxicity due to chronic alcohol intake, or cell proliferative diseases.
15 . A method for treating an individual for hypercholesterolemia, said method comprising: administering to said individual an amount effective to reduce the level of serum cholesterol, of a composite containing an edible biomass of Basidiomycetes, wherein the biomass includes a cholesterol-lowering compound.
16 . The method according to claim 15 , wherein said amount is effective to reduce the level of blood cholesterol from about 20% to 50%.
17 . The method according to claim 15 , wherein the biomass of Basidiomycetes is produced from fungi of a species of the genus Pleurotus.
18 . The method according to claim 17 wherein the species of the fungi is selected from the group consisting of Pleurotus ostreatus, Pleurotus eryngii var. ferulae, Hypsizygus marmoreus, Lepista nuda, Pleurotus cystidiosus, P. dijamor, P. pulmonarius, P. salignus, Grifola frondosa , and Hericium erinaeus
19 . The method according to claim 14 wherein the Basidiomycetes biomass is produced from Pleurotus eryngii var. ferulae CB 101938.
20 . The method according to claim 14 wherein the Basidiomycetes biomass is produced from Pleurotus ostreatus CB 101937.
21 . A nutrient medium suitable for growth of the Pleurotus biomass, the medium comprising of the following ingredients (g/l):
Medium A
Glucose (Dextrose)-
25
Peptone-
2
Yeast extract-
1
KH 2 PO 4-
0.5
MgSO 4 .7H 2 O—
0.25
CaCl 2 .2H 2 O—
0.1
Corn Steep Liquor-
2.5
ml
Trace Element Mixture
10
ml
10% KOH-
2.5
ml
Trace Element Mixture (g/l):
FeSO 4 .7H 2 O—
0.5
MnSO 4 .H 2 O—
0.1
ZnSO 4 .7H 2 O—
0.05
CuSO 4 .5H 2 O—
0.02
22 . A nutrient medium suitable for growth of the Pleurotus biomass, the medium comprising of the following ingredients (g/l):
Medium B
Glucose (Dextrose)-
50
Peptone-
0.5
Yeast extract-
1
(NH 4 ) 2 SO 4 —
2
K 2 HPO 4 3H 2 O—
2
K 2 HPO 4
2.5
Trace Element Mixture -
10
ml
Trace Element Mixture (g/l):
FeSO 4 .7H 2 O—
0.5
MnSO 4 .H 2 O—
0.1
ZnSO 4 .7H 2 O—
0.05
CuSO 4 .5H 2 O—
0.02Join the waitlist — get patent alerts
Track US2002137155A1 — get alerts on status changes and closely related new filings.
We store only your email — no account needed. See our privacy policy.