New annexin binding protein

Abstract

The invention provides a human annexin binding protein (NABP-1) and polynucleotides which identify and encode NABP-1. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of NABP-1.

What is claimed is:  
     
         1 . An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: 
 a) an amino acid sequence of SEQ ID NO:1,    b) a naturally-occurring amino acid sequence having at least 90% sequence identity to the sequence of SEQ ID NO:1,    c) a biologically-active fragment of the amino acid sequence of SEQ ID NO:1, and    d) an immunogenic fragment of the amino acid sequence of SEQ ID NO:1.    
     
     
         2 . An isolated polypeptide of  claim 1 , having a sequence of SEQ ID NO:1.  
     
     
         3 . An isolated antibody which specifically binds to a polypeptide of  claim 1 .  
     
     
         4 . A diagnostic test for a condition or disease associated with the expression of NABP-1 in a biological sample comprising the steps of: 
 a) combining the biological sample with an antibody of  claim 3 , under conditions suitable for the antibody to bind the polypeptide and form an antibody: polypeptide complex; and    b) detecting the complex, wherein the presence of the complex correlates with the presence of the polypeptide in the biological sample.    
     
     
         5 . The antibody of  claim 3 , wherein the antibody is: 
 (a) a chimeric antibody;    (b) a single chain antibody;    (c) a Fab fragment;    (d) a F(ab′) 2  fragment; or    (e) a humanized antibody.    
     
     
         6 . A composition comprising an antibody of  claim 3  and an acceptable excipient.  
     
     
         7 . A method of diagnosing a condition or disease associated with the expression of NABP-1 in a subject, comprising administering to said subject an effective amount of the composition of  claim 6 .  
     
     
         8 . A composition of  claim 6 , wherein the antibody is labeled.  
     
     
         9 . A method of diagnosing a condition or disease associated with the expression of NABP-1 in a subject, comprising administering to said subject an effective amount of the composition of  claim 8 .  
     
     
         10 . A method of preparing a polyclonal antibody with the specificity of the antibody of  claim 3  comprising: 
 a) immunizing an animal with a polypeptide of SEQ ID NO:1 or an immunogenic fragment thereof under conditions to elicit an antibody response;  
 b) isolating antibodies from said animal; and  
 c) screening the isolated antibodies with the polypeptide thereby identifying a polyclonal antibody which binds specifically to a polypeptide of SEQ ID NO:1.  
 
     
     
         11 . An antibody produced by a method of  claim 10 .  
     
     
         12 . A composition comprising the antibody of  claim 11  and a suitable carrier.  
     
     
         13 . A method of making a monoclonal antibody with the specificity of the antibody of  claim 3  comprising: 
 a) immunizing an animal with a polypeptide of SEQ ID NO:1 or an immunogenic fragment thereof under conditions to elicit an antibody response;  
 b) isolating antibody producing cells from the animal;  
 c) fusing the antibody producing cells with immortalized cells to form monoclonal antibody-producing hybridoma cells;  
 d) culturing the hybridoma cells; and  
 e) isolating from the culture monoclonal antibody which binds specifically to a polypeptide of SEQ ID NO:1.  
 
     
     
         14 . A monoclonal antibody produced by a method of  claim 13 .  
     
     
         15 . A composition comprising the antibody of  claim 14  and a suitable carrier.  
     
     
         16 . The antibody of  claim 3 , wherein the antibody is produced by screening a Fab expression library.  
     
     
         17 . The antibody of  claim 3 , wherein the antibody is produced by screening a recombinant immunoglobulin library.  
     
     
         18 . A method for detecting a polypeptide of SEQ ID NO:1 in a sample comprising the steps of: 
 a) incubating the antibody of  claim 3  with a sample under conditions to allow specific binding of the antibody and the polypeptide; and    b) detecting specific binding, wherein specific binding indicates the presence of a polypeptide of SEQ ID NO:1 in the sample.    
     
     
         19 . A method of purifying a polypeptide of SEQ ID NO:1 from a sample, the method comprising: 
 a) incubating the antibody of  claim 3  with a sample under conditions to allow specific binding of the antibody and the polypeptide; and    b) separating the antibody from the sample and obtaining purified polypeptide of SEQ ID NO:1.