Vitamin B metabolism proteins

Abstract

This invention relates to an isolated nucleic acid fragment encoding a vitamin B6 metabolic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the vitamin B6 metabolic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the vitamin B6 metabolic enzyme in a transformed host cell.

What is claimed is:  
     
         1 . An isolated nucleic acid fragment encoding a pyridoxal kinase comprising a member selected from the group consisting of: 
 (a) an isolated nucleic acid fragment comprising at least 400 contiguous nucleotides wherein the nucleic acid fragment encodes an amino acid sequence that is at least 80% identical to the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8;    (b) an isolated nucleic acid fragment that is complementary to (a).    
     
     
         2 . The isolated nucleic acid fragment of  claim 1  wherein nucleic acid fragment is a functional RNA.  
     
     
         3 . The isolated nucleic acid fragment of  claim 1  wherein the nucleotide sequence of the fragment comprises the sequence set forth in a member selected from the group consisting of SEQ ID NO: 1, SEQ ID NO:3, SEQ ID NO:5 and SEQ ID NO:7.  
     
     
         4 . A chimeric gene comprising the nucleic acid fragment of  claim 1  operably linked to suitable regulatory sequences.  
     
     
         5 . A transformed host cell comprising the chimeric gene of  claim 4 .  
     
     
         6 . An isolated nucleic acid fragment encoding a pyridoxamine-phosphate oxidase comprising a member selected from the group consisting of: 
 (a) an isolated nucleic acid fragment encoding an amino acid sequence that is at least 80% identical to the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14 and SEQ ID NO:16;    (b) an isolated nucleic acid fragment that is complementary to (a).    
     
     
         7 . The isolated nucleic acid fragment of  claim 6  wherein nucleic acid fragment is a functional RNA.  
     
     
         8 . The isolated nucleic acid fragment of  claim 6  wherein the nucleotide sequence of the fragment comprises the sequence set forth in a member selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13 and SEQ ID NO:15.  
     
     
         9 . A chimeric gene comprising the nucleic acid fragment of  claim 6  operably linked to suitable regulatory sequences.  
     
     
         10 . A transformed host cell comprising the chimeric gene of claim  9 .