Phosphoserine phosphatase gene of coryneform bacteria

Abstract

The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.

What is claimed is:  
     
         1 . A protein defined in the following (A) or (B): 
 (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or    (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.    
     
     
         2 . A DNA coding for a protein as defined in the following (A) or (B): 
 (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or    (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.    
     
     
         3 . A DNA coding for a protein having phosphoserine phosphatase activity, which is encoded by a DNA sequence which hybridizes under stringent conditions to a DNA sequence encoding SEQ ID NO: 2.  
     
     
         4 . The DNA of according to  claim 3 , the stringent conditions comprise washing at 60° C. and at a salt concentration corresponding to 1× SSC and 0.1% SDS.  
     
     
         5 . The DNA according to claim 2, which is DNA as defined in the following (a) or (b): 
 (a) A DNA which comprises a nucleotide sequence corresponding to nucleotide numbers of 210-1547 of nucleotide sequence of SEQ NO: 1 in Sequence Listing; or    (b) A DNA which is hybridizable with a probe which comprises the nucleotide sequence corresponding to nucleotide numbers of 210-1547 of nucleotide sequence of SEQ NO: 1. in Sequence Listing or a partial sequence of the nucleotide sequence under stringent conditions, and which codes for the protein having the phosphoserine phosphatase activity.    
     
     
         6 . The DNA of according to  claim 5 , the stringent conditions comprise washing at 60° C. and at a salt concentration corresponding to 1× SSC and 0.1% SDS.